الفهرس 
AcknowledgementOnly 14 pages are availabe for public view
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Abstract
This study was conducted to determine the prevalence of food poisoning pathogens in 150 raw milk samples collected from dairy farms ,farmers’ houses and dairy shops, 50 samples each in Assiut Governorate, Egypt.
E. coli, Staph. aureus and L.monocytogenes were detected in 32, 26, and 4 % of samples collected from dairy farms, and in 30, 32 and 6 % of farmers’ houses samples, while they were detected in 14, 22 and 4 % of dairy shops samples.
26.32% of the isolated E.coli was found to harbour the Stx1 gene while none of the isolates harbour Stx2.
The ability of Staph. aureus to produce enterotoxins which is linked to staphylococci enterotoxins SEs genes was investigated by using real time PCR, out of 40 Staph. aureus isolates, only 5 isolates were carrying Sea gene, while none had Seb, Sec, Sed or See genes.
The conventionally isolated L.monocytogenes were subjected to real time PCR for amplification of 16SrRna. Only 4 isolates were confirmed to be L.monocytogenes.
The suggestive hygienic measures to improve the quality of raw milk were discussed.