الفهرس 
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Abstract
Mesenchymal stem cells hold great promise for clinical applications in regenerative medicine. MSCs represent good source of multipotent somatic stem cells. Given the appropriate microenvironment, MSCs could differentiate into wide variety of cells. UC appears to be a good source of MSCs for a number of reasons; it is considered a medical waste, and therefore their use in research has a little ethical concern. UC MSCs proliferate rapidly in culture and are thought to be immune privileged. Harvesting cells from the UC is non-invasive procedure and can be performed in a comfortable manner without risk to the donor. We isolated MSCs from 100 UCWJ between November 2018 and June 2019, 79 of them were included in the final analysis. All babies were delivered by Caesarean section at the obstetric department of Mansoura University Hospital. When analyzing maternal variables, we found that maternal age can also significantly affect the MSCs count. In our study, increased maternal age was associated with decrease in MSCs count. P1 doubling time had a strong positive correlation with maternal age (r = 0.827, p <0.001) and a moderate positive correlation with gravidity (r = 0.624, p < 0.001) and parity (r = 0.551, p < 0.001). We found a weak positive correlation between MSCs yield and UC width (r = 0.285, p = 0.011). MSCs count was not statistically significant different between babies with cephalic presentation and those with abnormal presentation (p = 0.373). Presence of Meconium stained amniotic fluid was not associated with statistically significant difference in MSCs yield (p = 0.308). Preeclampsia toxemia and gestational diabetes did not have effect on MSCs yield and doubling time. We found that MSCs count was significantly affected by the baby’s birth weight. Higher birth weight led to higher cell count. Also, gestational age had significant effect on MSCs yield, increased gestational age led to higher MSCs count. Gender, birth length, and head circumference have no effect on MSCs yield. No significant correlation was found between P1 doubling time and gestational age (p = 0.186), or birth weight (p = 0.735).