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العنوان
Serological and Molecular survey on Toxoplasma gondii Infection in Small Ruminants in Sohag Governorate, Egypt /
المؤلف
Bakheet, Abeer Ahmed
هيئة الاعداد
باحث / عبير أحمد بخيت الديب
مشرف / أحمد محمود عطية
مناقش / أحمد كمال دياب
مناقش / أحمد عبد الراضي محمود
الموضوع
Animals - Diseases.
تاريخ النشر
2020.
عدد الصفحات
57 p.:
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Veterinary (miscellaneous)
الناشر
تاريخ الإجازة
23/4/2020
مكان الإجازة
جامعة أسيوط - كلية الطب البيطري - Veterinary Medicine
الفهرس
Only 14 pages are availabe for public view

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from 73

Abstract

Sheep and goats are important to the economy of many countries because they are a source of food for humans. Sheep and goats are commonly infected with the protozoan parasite, Toxoplasma gondii.
Infection with the parasite may cause early embryonic death and resorption, fetal death and mummification, abortion, stillbirth, and neonatal death. Severity of infection is associated with the stage of pregnancy at which the ewe becomes infected, the earlier in gestation, the more severe the consequences.In this study we collected 250 Ewe’s samples and 150 goat samples randomly from different areas in Sohag Governorate and were tested for toxoplasmosis by using LATEX agglutination test, (ELISA) and the aborted samples were tested by molecular tool (PCR) in small ruminants.
Prevalence of Toxoplasma gondii of the serologically tested sheep and goats were 45.83 % and 35.42 % respectively. Latex agglutination test (LAT, Card test) is a simple, cheapest and feasible tool for serological diagnosis of Toxoplasma gondii infection. ELISA technique is superior in serodiagnosis of Toxoplasma gondii infection in flocks. Detection of Toxoplasma gondii IgG rather than Toxoplasma gondii IgM is a predicting value to the chronic nature of the disease among the investigated sheep and goats. Toxoplasma gondii infection should be suspected clinically when woolless or hairless lambs of goats-kids with enlargement of heads of the aborted fetuses in the flocks.
Aborted fetuses must be burnt as soon as possible to avoid carnivorophages (wild and domestic cats) that completing of the Toxoplasma gondii cycle.
It can be concluded that ELISA combined with the PCR technique is a recommended tool for accurate diagnosis of Toxoplasmosis but PCR is more specific for detection of T. gondii when taking the ELISA as a reference test.