الفهرس | Only 14 pages are availabe for public view |
Abstract >This study recorded that a total of 200 table eggs samples (Farm and Baladi eggs) were randomly collected from poultry farms, markets, supermarkets, groceries and street- vendors in Beni-Suef city, Egypt. 100 farm eggs shell and content samples (each of 50) and 100 baladi eggs shell and content samples (each of 50) were examined for the presence of Coliforms, E. coli, Salmonella, coagulase positive Staphylococcus aureus and Staphylococcal enterotoxins. I- Isolation of Coliforms and E.coli: It was noted that Coliforms were contaminating 22 (11%) isolates of the examined Baladi and Poultry farm eggs. Out of them 20 (40%) and two (4%) Baladi eggs samples and Poultry eggs samples were contaminated by Coliforms, respectively. The total 100 shells samples of both Baladi and Poultry farm eggs were contaminated with Coliforms by 19%, while three (6%) content samples of the Baladi eggs were contaminated with Coliforms. The identified genera were Citrobacter freundii, Citrobacter diversus, E.coli (Atypical type), Edwardsiella tarda, Mogonella morganii, Klebsiella oxytoca, Providencea spp., Serratia fonticola and Yersinia intermedia with the incidences of (11.76%), (5.88%), (29.4%), (11.76%), (5.88%), (5.88%), (5.88%), (11.76%) and (11.76%) from Baladi eggs shell samples, respectively, while E. coli (Atypical type) and Providencea spp. were present in the contents of the Baladi eggs samples by (33.33%) and (66.67%), respectively. On the other hand, only two (100%) Enterobacter species were isolated from the shell samples of the examined poultry farm eggs. II- Isolation of Salmonella: Salmonella spp. could not be isolated neither from the Baladi nor the Poultry farm eggs in the current study. III- Isolation of Staph. aureus and Staphylococcal enterotoxins: In the present study a total of 13 (6.5%) isolates were identified as Staphylococcus spp. Out of them, 8 (61.5%) were coagulase positive Staphylococcus spp. and 5 (38.5%) were coagulase negative Staphylococcus spp. Coagulase positive Staph. aureus was revealed from 10% and 6% of Baladi table eggs samples and Poultry farm table eggs samples, respectively. Staph. aureus was reported in shells of Baladi eggs samples (6%) more than Poultry farm eggs samples (2%), while the egg contents of the examined eggs samples were positive for Staph. aureus strains equally in both Baladi and Poultry farm eggs samples (2%). All (100%) the examined Staph. aureus strains were positive for Staph. aureus CoA gene. The occurrence of classical enterotoxin genes (SEA, SEB and SED) in the present study was detected in 37.5 %, 0 and 25%, respectively. One sample (12.5%) was positive for both SEA and SED genes. |