الفهرس | Only 14 pages are availabe for public view |
Abstract Isolation and identification of some inulinolytic yeasts isolated from rhizospheric region of jerusalem artichoke plants. Studying inulinase production by the active yeast isolates using chicory roots, dahlia tubers, jerusalem artichoke tubers and garlic bulbs. Studying factors affecting inulinase production by the yeast isolates. Studying factors affecting activity of inulinase produced by the yeast isolates. four active isolates were selected and identified. The isolates were identified and belonged to Scopulariopsis brevicaulis, Sarocladium kiliense, Galactomyces candidum and Candida catenulata. The highest value of inulinase produced by Scopulariopsis brevicaulis (67.07µg/ml) was obtained after 48 hrs of incubation at 30°C on medium contained jerusalem artichoke tubers, yeast extract, sucrose, Inoculum size 6% and initial pH 5.0. While the highest value of inulinase produced by Sarocladium kiliense (60.19µg/ml) was obtained after 48 hrs of incubation at 30°C on medium contained dahlia tubers, yeast extract, maltose, Inoculum size 4% and initial pH 6.0. As for, the highest value of inulinase produced by Galactomyces candidum (46.59µg/ml) was obtained after 48 hrs of incubation at 30°C on medium contained jerusalem artichoke tubers, yeast extract, fructose, Inoculum size 6% and initial pH 7.0. While the highest value of inulinase produced by Candida catenulata (67.52µg/ml) was obtained after 48 hrs of incubation at 25°C on medium contained dahlia tubers, yeast extract, maltose, Inoculum size 6% and initial pH 4.0.. |