الفهرس 
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Abstract
Examination of 144 environmental samples collected from Helwan
University revealed that free-living amoebae (FLAs) were detected in 91.7%
of irrigation water samples, 83.3% of soil samples, 54.2% of swabs samples
from surfaces, 50% of domestic waste water samples and lastly tap water and
air samples have the same percentage 12.5%.
Concerning seasonal variations, the highest occurrence of free-living
amoebae in irrigation water samples was recorded in spring and summer
(100% in each), followed by the same percentage in winter and autumn
(83.3%).
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Concerning soil samples, the highest occurrence of free-living amoebae
was recorded in spring (100%), followed by 83.3% in each of summer and
winter and 66.7% in autumn.
The highest occurrence of free-living amoebae in swabs from surfaces
samples was recorded in spring (100%), followed by summer (50%), winter
and lastly in autumn (16.7%).
The occurrence of free-living amoebae was the same in spring, autumn
and winter (represented by 16.7% for each), while they disappeared in
summer.
Six genera of FLAs (Acanthamoeba, Naegleria, Vahlkamfia,
Vermamoeba, Vannella and Saccamoeba) were isolated from collected
environmental samples Helwan University.
The morphologically identified FLAs were subjected to molecular
confirmation by simple peR techniques using genus-specific primers for the
detected genera.