الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Hyaluronidase enzyme (HAase) catalyses the hyaluronic acid (HA) and plays a role in maintaining the healthy state of the skin. Bioassay-guided fractionation of Ravenala madagascariensis (Sonn.) organ extracts (leaf, flower, stem and root) by testing their hyaluronidase inhibition was performed followed by metabolic profiling using LC-HRESI/MS. Additionally, a hyaluronidase docking study was achieved using Molecular Operating Environment (MOE). Results revealed that the total crude hydroalcoholic extract (70% EtOH) and the n-BuOH fraction of the leaves showed high HAase activity with 90% and 64.3% inhibition, respectively. Metabolic analysis of Ravenala madagascariensis resulted in the identification of ninteen phenolic compounds ranging from different chemical classes (flavone glycosides, flavonol glycosides and flavanol aglycones). Bioassay-guided purification of the leaf n-BuOH fraction led to the isolation of seven compounds that were identified as narcissin, rutin, epiafzelechin, epicatechin, isorhamnetin 7-O-glucoside, kaempferol and isorhamnetin-7-O-rutinoside. The docking study showed that narcissin and rutin interact with HAase through hydrogen bonding with the Asp111, Gln271 and/or Glu113 residues. Our results highlight Ravenala madagascariensis and its flavonoids content as promising hyaluronidase inhibitors in natural cosmetology preparations for skin care.