الفهرس 
Acknowledgements
Molecular methods for identification of mycoplasmasOnly 14 pages are availabe for public view
 |  | from | 109 |  |  |
| | | from | 109 | | |
Abstract
Contagious caprine agalactiae is a highly contagious disease of goats caused by Mycoplasma agalactiae bacteria. The disease can spread rapidly through the herd by auricular, ocular and nasal secretion, faeces, urine, milk and excretions from joint lesions as well as sexual transmission through infected male.
Mycoplasma cause significant economic losses due to the loss of milk and loss of kids because of abortions, neonatal deaths, and loss of animals themselves.
The present study was applied on 180 balady goats at Qena governorate for showing the following:
1- The clinical signs that appear on goat infected with Mycoplasmaagalactiae.
2- Isolation and identification of Mycoplasma agalactia by culture and PCR, and isolation of other mastiticbacteria.
This study cleared that:
1- The percentage of clinical infected goats by Mycoplasma agalactiae was 31.1%.
2- The most clinical observation noticed on infected goats with Mycoplasma agalactiae were the transient fever, mastitis, arthritis, andkeratoconjunctivitis.
3- Isolation and identification of Mycoplasma agalactiae by culture and PCR revealed that the percentage of Mycoplasma agalactiae on collected sample was 32.14% alone or joined with Staphylococcus aureus and Trueperellapyogenes.
4- The accuracy and sensitivity of PCR in the detection of Mycoplasma agalactiae was more than the cultural methods. No culture methods positive samples were negative inPCR.