الفهرس 
Red Blood Cell Antigens and AntibodiesOnly 14 pages are availabe for public view
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Abstract
Background: In multitransfused patients, extended blood group genotyping overcomes the limitations of serological method & assists in the selection of red blood cells (RBCs) units for transfusion that leads to better in vivo RBC survival and diminishes frequency of alloimmunization.
Aim of the work: The goal of this study was to describe a PCR methodology to identify the polymorphisms of the Kell, Duffy and Kidd blood group systems and to compare RBC molecular genotyping to serological phenotyping in chronically transfused patients
Methods: Kell, Kidd & Duffy phenotyping & genotyping by RFLP were done for 100 repeatedly blood transfused patients
Results: Serological identification of minor blood groups revealed that in Kidd blood group the most common antigen pattern is JK (a+b-) being 34%, in Duffy FY (a+b-) being 26% &for Kell; 96% were k antigen positive & 92% were K antigen negative. As regard genotyping, the most frequent genotypes of the Kell, Kidd, Duffy systems were KEL02/KEL02 (98%), JK01/JK02 (42%) and FY02/FY02(60%) respectively. Discrepancy between genotyping & phenotyping was also found in 76% of patients in the Kidd system, 92% of patients in Duffy system & 2% in Kell blood group system.
Conclusion: Molecular genotyping is superior than serological analysis to identify the minor RBC Ags Kell, Kidd & Duffy in repeatedly transfused patients.