Search In this Thesis
   Search In this Thesis  
العنوان
Biochemical studies on aflatoxins in some industrial foods /
المؤلف
Abbas, Mohamed Shebl Sadek.
هيئة الاعداد
باحث / محمد شبل صادق عباس
مشرف / سامى طلعت أبوطالب
مشرف / أحمد محمد يوسف
مشرف / مصطفى إبراهيم سند
مناقش / محمد شعبان رفاعي
مناقش / رمضان أحمد حسن
الموضوع
Agricultural Chemistry. Antioxidant Activity. Industrial Foods.
تاريخ النشر
2020.
عدد الصفحات
online resource (152 pages) :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
الزراعية والعلوم البيولوجية (المتنوعة)
تاريخ الإجازة
1/1/2020
مكان الإجازة
جامعة المنصورة - كلية الزراعة - Department of Agric. Chemistry
الفهرس
Only 14 pages are availabe for public view

from 152

from 152

Abstract

The present study was carried out using seeds for one of medicinal plants namely: Garden cress, (Lepidium sativum), belongs to family Brassicaceae. Seeds were purchased from the Agricultural Research Center, Giza, Egypt. Aflatoxin B1 was determined in some foodstuffs such as Biscuits, chips, luncheon,roomy cheese and folia sweetmeat. Four extracts were obtained from the plant seeds by successive extraction for crude methanol extract which named petroleum ether, chloroform, ethyl acetate and methanol extracts. chemical analysis were determined as following: Determination of aflatoxin B1 in some foodstuffs. Total polyphenols content of Lepidium sativum extracts. Total flavonoids content of Lepidium sativum extracts- The effect of lepidium sativum seed extracts on Aspergillus flavus:- Determination of Antioxidant Activity Using (DPPH) Method. Methods and Results: Aflatoxin B1 was detected in biscuits, chips, luncheon, Roomy cheese and Folia sweetmeat, which were 0.9, 7.0, 0.7, 1.28 and 6.8 ppb, respectively. Aflatoxin B1 was represents a highly level in both chips and Folia sweetmeat, while lowest level in Luncheon, Biscuits and Roomy cheese. Lepidium sativum seeds of methanolic extract have the highest concentration of total polyphenols, which was 9.11 mg GAE/g (dry weight). While, ethyl acetate extract have the lowest concentration of total polyphenols, which was 4.44 mg GAE/g. On the other hand, Lepidium sativum seeds of chloroform and petroleum ether extracts contained medium values of total polyphenols which were represent 8.77 and 7.22mg GAE/g, respectively. Chloroform extract contained the highest amount of total flavonoids which was 6.02 mg QE/g dry weight, while ethyl acetate extract contains the lowest amount 3.77 mg QE/g dry weight. On the other hand, methanol and petroleum ether extracts gave 5.59 and 5.70 mgQE/g dry weight, respectively. Chloroform extract of lepidium sativum seeds revealed the highest growth inhibition which ranged from (38 to 96%) against Aspergillus flavus at concentration ranged from 1000 to 5000 ppm. The highest growth inhibition for Chloroform, methanol, ethyl acetate and pet. ether extracts at the concentration of 5000 ppm were represented 96,26.5,23.4 and17.8% respectively. While, the percentages of moderate growth inhibition for methanol, ethyl acetate and petroleum ether extracts were 16.7, 12 and 8.3% at the concentration of 3000ppm, respectively. The inhibition percentage of chloroform and methanol extracts for Lepidium sativum seeds have the most effective of inhibition percentage (53 to 82% and 48 to 78%) at the concentrations of 125 and 1000 mg/ml, respectively. Ethyl acetate and pet-ether extracts were represented the moderate and lowest values, respectively. The best results of IC50 was represented the value of 470.36 mg/ml for chloroform extract.