![]() | Only 14 pages are availabe for public view |
Abstract This work was planned to investigate the potential effect of selenium chitosan nanoparticles and omega 3 chitosan nanoparticles separately in Nile tilapia fingerlings. Two hundred forty Nile tilapia fingerlings were used in each experimental investigation of this study. The fish were obtained from a local fish hatchery (Central laboratory for aquaculture research, Abbassa, Abu-Hammad, Sharkia , Egypt). 1) Experiment I: The fish were divided into equal 4 triplicate groups (each replicate contained 20 fish) and each replicate of the fish groups was stocked in its corresponding glass aquarium for two weeks to be acclimatized before the start of the experiment. Two hundred forty Nile tilapia fingerlings were assigned into four equal groups: group 1: Control diet without se-chitosan NPs. group 2: Control diet + se-chitosan NPs (0.25mg/kg). group 3: Control diet + se-chitosan NPs (0.5 mg/kg). group 4: Control diet + se-chitosan NPs (1 mg/kg). At the end of experimental period (12 weeks) blood samples were collected from caudal vein, centrifuged at 3000 r.p.m for 15 minutes, the serum samples are separated and store - d at -20◦C and used for estimation of biochemical parameters. |