الفهرس 
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Abstract
This study included 50 human and 20 dog having upper gastrointestinal symptoms those underwent for endoscopy and subsequently diagnosed as patients with gastritis, peptic ulcer and non-ulcer dyspepsia as group 1. Another 55 patients having gastric injury/tumor and 20 dogs without upper gastrointestinal symptoms were included as group 2. At the time of endoscopy, 2 biopsy specimens were collected from each of group 1 patients and dogs for culture and histopathological examination. Two biopsy specimens were collected from each of group 2 patients for molecular diagnosis and immunohistochemistry. Serum samples from both groups were tested for anti-H. pylori IgA by ELISA. Stool samples from both groups were tested for H. pylori antigen by rapid test. Serological identification for H. pylori Antigen and antibodies reveals (66% human, 45% dogs) and (88% human, 85% dogs) from group 1 and (63% human, 50% dogs) and (87% human, 90% dogs) from group 2, respectively. All stool samples from group 1 undergo molecular diagnosis to detect H. pylori DNA and the result was positive in 27 of 50 human specimens with percentage 54% and 7 of 20 dogs’ specimen with percentage 35%. The best recovery for primary isolation of H. pylori from gastric biopsy specimens from group 1 was from skim-milk with 17% glycerol transported media (15 of 70 samples 21.4%) (12 human, 3 dogs) plated on Pylori agar (PA) and followed skim-milk with 17% glycerol transported media (14 of 70 samples 20%) (10 human, 4 dogs) plated on Columbia blood agar (CBA) then Brucella broth with 17% glycerol (9 of 70 samples 12.8%) (7 human, 2 dogs) and plated on PA. Skim milk broth was the best media used for storage of H. pylori for 2 weeks at -30⁰c the recovery rate was 20% when plated on PA and CBA. Out of the 50 human biopsy specimens, total of 44 biopsies were identified by histopathology to have inflammation and H. pylori. Genotyping of H. pylori based on 16S rRNA gene show 54.5% of human and 35% of dogs were positive from group 2. Immunohistochemistry revealed strong expression of CD44 in H. pylori- associated gastric cancer cases, MMP-2 expression was observed in all neoplastic lesions associated with H. pylori infection.