الفهرس 
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Abstract
Coronary heart disease is supposed to be the leading cause of death in developing countries by the year 2020. In Egypt, mortality due to CHD is rapidly rising to be one of the highest mortality rates worldwide. The high prevalence rate could be due to risk factors (hypertension, dyslipidemia and obesity) which are common among Egyptians. In 2011 CAD deaths in Egypt reached 21.73% of total deaths due to CAD around the world.
This is a case control analytical study conducted on 100 individuals at Suez Canal University Hospital, Ismailia General Hospital and Ismailia Insurance Hospital Cardiology premises (clinics and inpatient department) trying to find a correlation between the platelet ADP receptor (P2Y12) polymorphism and coronary heart diseases’ patients in Ismailia.
Target population (equal to 100 individuals) were recruited randomly from the fore mentioned settings and divided into two groups, a Study group (50 patients) who were diagnosed with CHDs or its variants, either Patients ever diagnosed with myocardial infarction (MI) or patients who have >1 cardiovascular diseases risk factors (hypertension, diabetes mellitus, history of CHDs, dyslipidemia, and/or smoking). The control group individuals (50 participants) are of apparently normal and healthy subjects attending for routine medical check-up and/or blood donation.
The patients were subjected to Interview Questionnaire, Blood sampling (of 7ml fresh blood) for Laboratory Investigations, DNA extraction and examination for P2Y12 gene polymorphism. Genotyping of ADP receptor (P2Y12) for gene polymorphism was done starting with (PCR)-(RFLP) analysis to detect the P2Y12 gene polymorphisms using specific primers and RsaI (Afa I) restriction master reaction.