الفهرس 
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Abstract
The present study aimed to rapid detection of FAdV by conventional PCR technique. 250 samples were collected from different 50 broiler farms in Egypt and different organs (liver, lung, kidney, spleen, heart fluid, cloacal swab) were collected from clinically diseased broiler chicken. Viral DNAs were extracted from these clinical tissue samples and then conventional PCR was performed by using hexon gene. Histopathology also performed on liver which preserved in 10% formalin that depends on the presence of eosinophilic or basophilic intranuclear inclusion bodies in hepatocyte. A trial for isolation of FAdV from conventional PCR positive samples via CAM of 9-11 days ECEs was conducted. Four positive samples from fifty flocks were detected by PCR according to the hexon gene with expected product size of 890 bp. Sequencing analysis revealed fowl adenovirus type D serotype 11. Histopathology showed basophilic intranuclear inclusion body hepatitis in hepatocytes. The results of isolation of positive PCR results revealed difference in embryo mortality and some lesions as congestion and haemorrhage of embryo and its liver appeared swollen with focal necrosis and petechial & ecchymotic haemorrhages. CAM had congestion, haemorrhage and opacity at 5th day postinoculation. The pathogenicity test showed that experimentally infected chicks give negative histopathological and PCR results may be due to immunity of breeder.