الفهرس 
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Abstract
Iron-deficiency anemia is another global nutritional problem occurring as a complication of nutritional and absorption disorders and is observed frequently over ages. Shortage in dietary iron intake or absorption represents the major risk factor of the incidence of iron-deficiency anemia. Iron-deficiency has been strongly related with many human diseases including immune disorders, chronic inflammation, restriction of physical performance, neurological impairment and cognitive deficits.
Lagenaria siceraria (molina) contains various phytochemical constituents like, triterpenoid, cucurbitacins, flavones, vitamin B, beta glycosides and fair source of ascorbic acid in fruit pulp. The plant has been studied for their various pharmacological activities like, antioxidant, cardioprotective, diuretic, immunomodulatory effects, hepatoprotective, lipid peroxidation and anti-inflammatory activity.
This study aimed to investigated the effect of molina fruit pulp and its seeds on biological parameters of anemic rats. Thirty six adult albino rats of an average weight (120±5g) and age (3 months) were used in this study. Rats cages kept in a room at temperature 25◦ C and under normal healthy conditions. All rats fed on a basal diet for 7 days to make adjustment then thirty rats fed on diet free in iron and vitamin C for two weeks to induce anemia . Thirty six rats divided into six groups, the first group fed on basal diet and the other five groups ( anemic rats ) were fed on the tested diets as the following:
group 1: Rats fed on basal diet as a control group.
group 2: Anemic rats fed on basal diet free from iron and vitamin C as a positive control group.
group 3: Anemic rats fed on basal diet free from iron and vitamin C with 2.5% of Lagenaria siceraria fruit pulp powder.
group 4: Anemic rats fed on basal diet free from iron and vitamin C with 5% of Lagenaria siceraria frui pulp powder.
group 5: Anemic rats fed on basal free from iron and vitamin C with 2.5% of Lagenaria siceraria seeds powder.
group 6: Anemic rats fed on basal diet free from iron and vitamin C with 5% of Lagenaria siceraria seeds powder.
At the end of experiment (28 days) blood samples were collected after 12 hours fasting and serum was separated for determination of blood glucose, hematological indices (RBC, HGB, PLT,WBC, Lymph), lipids profile include determination of serum total cholesterol, serum triglycerides, total lipids, high density lipoprotein (HDL-c), calculation of very low density lipoprotein cholesterol (VLDL-c), calculation of low density lipoprotein cholesterol (LDL-c) were examined and calculation of atherogenic index (AI), albumin, liver functions (ALT- AST and ALP), kidney functions (creatinine, uric acid and urea) for anemic rats.
At the same time, the organs: Heart , liver, kidney, lungs, and spleen were removed, washed in saline solution, dried by filter paper, weighted, while liver and kidney stored frozen in formalin solution 10% for histopathological testing. The data were statistically analyzed using computerized SPSS .