الفهرس 
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Abstract
There are two routes of contamination of blood stored for transfusion either endogenous (the source is the donor) or exogenous route (contamination can happen during the withdrawal and preparation)
It is important to note bacterial contamination of the stored blood preserved for transfusion is a significant problem with multiple causes. Most are related to contamination of the blood bags as a result of improper skin disinfection during blood collection. We strongly recommend that infection control rules must be strict and strongly supervised all the time. However, if the source is occult bacteremia in the donor, careful selection and questionnaire fulfill will reduce the problem.
The goal of this study is to detect the prevalence of bacterial contamination of donated blood before transfusion.
A specimen obtained from the Blood Bank of Menoufia University Hospitals, One hundred units were collected from Menoufia University Hospitals. Seventy five packed red cells and twenty five platelet units were examined. All platelet concentrates were taken on the fifth day of donation. Microbial contamination was detected by culture and PCR. The results were as following:
from the 100 refrigerated donor blood, 10 blood bags representing 10% showed bacterial contamination by PCR.
Culture showed eight percent bacterial isolates. Six of the isolates Gram-positive cocci representing 6% of total units, so it the commonest isolate. 4(4%) were gram-positive cocci were detected to be coagulase-negative staphylococci and 2 (2%) were Staphylococcus aureus. There were 2(2%), which were Gram-negative rods; one was identified as Escherichia coli and the other one was Klebsiella pneumonia .
Among gram positives Staphylococcus aureus and Coagulase negative Staphylococcus of the isolates was sensitive to clindamycin, ciprofloxacin,gentamicin, doxycycline but coagulase negative staph was resistant to cefoxitin 2(50%), Staphylococcus aureus showed (100%) resistance cefoxitin , Penicillin, Majority of isolated Gram-negative bacteria isolates showed increased resistance to tetracycline. However, all gram-negative isolates showed 100% sensitivity to both ciprofloxacin and gentamycin
The diagnostic test performance for detection of results of PCR in comparison to culture was 100% sensitivity and 97.8% specificity. Two false positive and no false negative cases were detected.
The sensitivity and negative predictive value of PCR for the detection of any of the isolated organisms in reference to the culture was 100%.