الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 143 |  |  |
| | | from | 143 | | |
Abstract
Background: The current study was conducted to evaluate the role of dietary cholesterol on insulin resistance or even diabetes type 2 and the protective role of SFN on rats exposed to normal and HCF diets.This study has been applied on twenty-four male albino rats and their weight ranged from 100-125 grams for 15 weeks months of corrected gestational age using specific reference data. Type of study: prospective observational study. Materials and Methods: group (1) control group: This group was consisted of 6 rats that were fed on normal diet (table.1) For 15 weeks. group (2) control + SFN group: This group was consisted of 6 rats that were fed on normal diet (table.1) for 11 weeks then SFN was given with(0.5mg/kg/day) orally for another 4 weeks. group (3) HCF diet group: This group was consisted of 6 rats that were fed on normal diet (table.1) mixed with cholesterol 1% with 10% fructose solution (HCF) for 15 weeks. group (4) HCF + SFN group: This group was consisted of 6 rats that were fed on normal diet (table.1) mixed with cholesterol 1% with 10% fructose solution (HCF) for 11 weeks then SFN was given with(0.5mg/kg/day) orally for another 4 weeks. Results: the mean value of hepatic reduced glutathione in the group HCF only was significantly decreased. While in HCF+SFN group was increased significantly. SFN administration revealed a significant decrease of cells stained with caspase 9. HCF administration revealed a significant increase of serum total cholesterol. which, reversed by SFN treatment. Serum triacylglycerol level increase greatly in HCF and decrease after administration of SFN. By feeding HCF a significant decrease in the mean value of serum HDL occurs. followed by a significant increase after treatment with SFN. administration of HCF led to significant increase in serum LDL and after treatment with SFN, the mean value of serum LDL was significantly decreased. Conclusion: The results of the current study revealed that SFN alleviate the oxidative damage induced by high cholesterol diet in rats and improving antioxidant status (reduced glutathione), decreasing oxidative and nitrosative stress markers (malondialdehyde and nitric oxide), improving liver damage markers. Enhancing glycemic status and decreasing caspase 9 concentration. It can be concluded that SFN can be used efficiently as antidiabetic agent. Recommendations: It is recommended to use broccoli or SFN as an active substance to lower the hyperglycemia in patients with diabetes type 2 (antidiabetic agent).