الفهرس 
Introduction and Aim of the workOnly 14 pages are availabe for public view
 |  | from | 193 |  |  |
| | | from | 193 | | |
Abstract
This study aimed to identify some viruses affecting ducks by Serological and molecular studies.Twenty pooled liver samples were collected from ducklings less than 2 weeks showed nervous signs and high mortality suspected to be infected by duck hepatitis virus (DHV). All samples were inoculated in allantoic cavity of SPF embryonated chicken eggs (ECGs) for three successive passages. Isolates were serologically identified by agar gel precipitation (AGPT) and fluorescent antibody technique (FAT) by using DHAV-1 antibodies as well. Also, other 20 pooled lung and pancreas samples from ducks more than one month suspected to be infected by avian influenza virus (AIV) showed nervous signs; ataxia and torticolus. Each suspected AIV sample was divided into two parts, one part was collected on ether 0.2% then directly tested by hemagglutination test (HAT), the other part was collected on trizol and used for reverse transcriptase polymerase chain reaction (RT-PCR). Finally, RT-PCR used for detection of DHAV-1 3D gene and AIV H5 gene and sequencing analysis of obtained 3D and H5 gene sequences. Twelve out of 20 local isolates of DHAV-1 were positive in AGPT and 15 gave positive result in FAT. Twelve samples suspected to be AIV gave positive HA reaction. By RT-PCR 5 selected isolates of DHAV-1 and 5 out of 10 tested samples of AIV were positive and gave the expected bands. Our DHAV-1 isolates showed 100% identity to other Egyptian sequences from GenBank and H5 AIV showed 96-97% identity.