الفهرس 
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Abstract
Considering the Diabetes mellitus (DM) as one of the oldest conditions characterized in humans, it has been recognized since antiquity and considered as a major health problem at present. There is a need for efficient treatment. The aim of the present investigation was to find out whether epidermal growth factor can alleviate the well documented side effects of streptozotocin induced diabetes mellitus on the submandibular salivary gland of adult male Albino rats.
Materials and Methods:
Sixty adult male Albino rats, three months old and 200-220 gm body weight were used and divided into three groups where n=20 as follows:
group 1.1: consisted of 20 rats received single intraperitoneal injection of 1ml/kg sodium citrate buffer (STZ) solvent and served as controls.
group 2.1: consisted of 20 rats and subjected to induction of diabetes mellitus using streptozotocin injected intraperitoneally as a single dose of 60mg/Kg body weight after being freshly prepared by dissolving in cold 0.1 M sodium citrate buffer. Animals were not considered diabetic unless they demonstrated serum glucose level greater than 250 mg/dl.
group 3.1: consisted of 20 rats they were treated as group II animals. they were subjected to daily intraperitoneal injection of epidermal growth factor(Epidermal Growth Factor, human -animal component free- recombinant, expressed in E. coli) provided by Sigma-Aldrich, Inc. in a dose of 10 µg/Kg body weight for two months.
The sections from submandibular salivary gland of rats were processed and stained by hematoxylin and eosin stain for histological examination and immunohistochemical stain for localization of cytokeratin, myosin and E-Cadherin in the glandular tissue. Other small specimens one cubic mm were cut and processed for ultrastructural examination by TEM.