الفهرس 
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Abstract
Summary
In this study,
We attempted for the isolation, purification and identification of Actinomycetes species isolated from Wady El-Natron area in North Egypt which characterized by high alkalinity and high salinity and detecting the selective media for maximum enzyme production.
The present research is designed to extract the fibrinolytic enzyme from Actinomycetes sp. isolated from soil, those are able to produce fibrinolytic enzyme.
A total of 136 Streptomyces isolates were isolated from various 21 soil samples collected from three different lakes (white, green and red), from Wady El-Natron region in North Egypt, during January, 2014. By using different media for isolation and differentiation according to the key proposed by Kuster (1972) and Bergey’s manual (1989). The screening for active Streptomyces spp. for our work was done by using the selective media - starch nitrate agar (SNA) and differential media - milk clotting agar, casein hydrolytic agar and heated plasma agar for the screening of those producing fibrinolytic enzyme.
The screening of fibrinolytic activity by milk clotting assay is the primary method to show the presence of fibrinolytic enzymes (had ability to hydrolyze the fibrin in milk = making hole zone around or not). The extracted fibrinolytic enzyme showed more potent fibrinolytic activity, which is a clinical thrombolytic agent. So, the 136 Streptomyces isolates were tested on milk clotting agar plates, thus, they were narrowed down to 21 isolates based on their ability to produce the fibrinolytic enzyme or not.