الفهرس 
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Abstract
Helicobacter pylori chronically colonize gastroduodenal mucosa and induce gastroduodenal diseases. In order to clarify whether gastroduodenal diseases in dyspeptic patient whose undergo gastrointestinal endoscopy in Minia University Hospitals correlated to H. pylori and immunoregulatory B cells, this based prospective and this study were conducted from December 2018 up to June 2019.
A total of 112 subjects undergoing gastrointestinal endoscopy were enrolled in this study. And three biopsies were obtained for detection of H. pylori by urease test, culture, biochemical tests, and molecular diagnosis. For detection of Breg cells blood samples were taken from all patients
In this study 55/112 (49.1%) of H. pylori was detected. The most frequent clinical symptom of the patients was dyspepsia (67%), followed by heartburn (49.1%), and the least one was abdominal distension (9.8%). The specific diseases most commonly found on upper gastrointestinal endoscopy were gastritis which was the highest percentage (22.3%) followed by peptic ulcer and gastric malignancy (12.5% each). The 112 patients participating in this study were 69 males (61.6%) and 43 females (38.4%). Ulcerative and malignant lesions were found more common in males, whereas gastritis was common among female patients, which was statistically significant. It was revealed that ulcerative diseases most common in age group >60 years, while malignant conditions were greater in age group > 50 years old, but there was no significant relationship
The most frequent diagnosis was gastritis (29.1%). There was no significant relation between the H. pylori infection and the final diagnosis
All the isolates were resistant to Amoxicillin, Levofloxacin, and Rifampicin, (100%). While 53 (96.36%) isolates were resistant to Metronidazole. Between them, the resistant isolates to Clarithromycin, and Tetracycline were 54 (98.18%) isolates for both. Multiple Combination Bacteriocidal Testing (MCBT) show very high resistance rates.
The percentages of CD19+CD24⁺CD38+ B cells in all patients were evaluated. Bregs levels were significantly higher in H. pylori negative patients (p= 0.013), while total leukocytes count (TLC) showed no significant differences.
The relationship between Bregs levels and age groups were statistically significant (p= 0.004). Bregs levels were lower at the extreme age groups (less than 26 and more than 60). The relationship between Bregs levels and diagnosis groups showed no statistical significance. The highest Bregs levels were associated with the malignant conditions.