الفهرس 
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Abstract
Summary
This present study aimed to detect effect of PUVA on JAK/STAT pathway and its relationship with the pathogenesis of Vitiligo.
The study was a Case-Control study conducted from Abril 2019 to December 2019 in the Dermatology outpatient clinic at Beni-Suef Univeristy Hospital. This study included 30 patients that had Vitiligo disease distributed as 7 males and 23 females’ patients, their age ranged from 20 to 50 years, the average age was 33.73±8.7. And 30 healthy controls were taken, they were age and sex matched to the Vitiligo cases. All participants were subjected to full clinical and laboratory investigations. Skin biopsies had been taken from all studied participants (Vitiligo cases and healthy controls) to study effect of PUVA on JAK/STAT pathway and its relationship with the pathogenesis of vitiligo.
Written Informed consent had been taken from all studied participants prior to beginning of the study. All participants were subjected to full clinical and laboratory investigations. Skin biopsies had been taken from all studied participants (vitiligo cases and healthy controls) to study the expression of JAK/STAT pathway and its relationship with the pathogenesis of Vitiligo.
Our data analyses reveled that:
- Tissue expression of JAK was significantly higher in Vitiligo skin lesions as compared with healthy skin biopsies taken from controls; the mean JAK values (5.28 vs. 1.03) in Vitiligo skin lesions and healthy skin from controls respectively with a statistically significant p-value< 0.001. At the same time; tissue expression of STAT was significantly higher in Vitiligo skin lesions as compared with healthy skin biopsies taken from controls; the mean STAT values (5.92 vs. 1.01) in Vitiligo skin lesions and healthy skin from controls respectively with a statistically significant p-value< 0.001.
- Tissue expression of JAK was still significantly higher in Vitiligo skin lesions as compared with healthy skin from controls even after exposure to PUVA; the mean JAK tissue expression values (3.40 vs. 1.03) in Vitiligo skin lesions and healthy skin respectively with a statistically significant p-value< 0.001. Also; tissue expression of STAT was still significantly higher in Vitiligo skin lesions as compared with healthy skin from controls even after exposure to PUVA; the mean STAT tissue expression values (3.28 vs. 1.01) in Vitiligo skin lesions and healthy skin respectively with a statistically significant p-value < 0.001.
- Tissue expression of JAK decreased significantly after exposure to PUVA in Vitiligo skin lesions; the mean tissue expression values (5.28 vs. 4.40) before and after exposure to PUVA in Vitiligo skin lesions respectively with a statistically significant p-value< 0.001. Also; tissue expression of STAT decreased significantly after exposure to PUVA in Vitiligo skin lesions; the mean tissue expression values (5.92 vs. 3.28) before and after exposure to PUVA in Vitiligo skin lesions respectively with a statistically significant p-value< 0.001.
- Tissue expression of JAK was significantly higher in Vitiligo skin lesions in patients with positive as compared with negative family history; with a statistically significant (p-value= 0.011)
- A significant linear moderate positive correlation between tissue expression of STAT and patients’ age among studied Vitiligo patients; (r= 0.423, p=0.020).
- A significant linear moderate negative correlation between tissue expression of STAT and duration of last new lesion among studied Vitiligo patients; (r= -0.561, p=0.001).
- A significant linear moderate positive correlation between tissue expression of STAT and Vitiligo disease activity (VIDA) score among studied Vitiligo patients; (r= 0.547, p=0.002).
- A significant linear strong positive correlation between tissue expression of JAK and tissue expression of STAT among studied population; (r= 0.762, p<0.001),
We concluded that:
JAK/STAT levels in Vitiligo patients were higher than in normal controls and these levels were down regulated after treatment with PUVA. So we suggest that JAK/STAT plays an important role in the pathogenesis of the Vitiligo disease. This could also open a new era for treatment of Vitiligo disease by anti-JAK modalities.