الفهرس 
ABSTRACTOnly 14 pages are availabe for public view
 |  | from | 140 |  |  |
| | | from | 140 | | |
Abstract
Aim: The aim of our study to investigate and compare the effect of green, medium roasted
and decaffeinated coffee on the viability, apoptosis, cell cycle progression, proliferation, invasion
and migration of OSCC cell lines.
Methodology: Coffee extracts were prepared and oral squamous cell carcinoma cultures
were exposed to various concentrations of coffee extracts for 72 hr. Cell viability was assessed and
IC50 values for each extract were quantified using MTT assay. Using flowcytometry, apoptotic and
necrotic cell population were calculated followed by the assessment of caspase-3 and caspase-9
activity. Cell cycle analysis for each coffee extracts was conducted. Next, EGFRTK assay was done
to obtain the inhibition % of tyrosine kinase activity in the studied groups. We assessed the antimigration
and anti-invasion effect of coffee extracts on oral squamous cell carcinoma cells using
Transwell invasion and migration assay. Finally, HPLC analysis was performed to measure the
amount of total phenolic, chlorogenic acid and caffeine in coffee extracts.
Results: The results showed a presence of cytotoxic effect of coffee extracts and the IC 50
was 17.1±0.69, 57.95±2.28, and 103.32 ±4.52 in green coffee, roasted coffee and decaffeinated
coffee respectively. The total apoptotic and necrotic cell populations was increased in green coffee
followed by roasted then decaffeinated compared to control. Capsase-3 and caspase-9 activity was
up-regulated in all coffee extract groups with the same order. All coffee extracts arrested cell cycle
at G2-M phase. The greatest EGFRTK activity inhibition percentage was in green coffee followed
by roasted then decaffeinated. Green coffee exerted the highest migration and invasion inhibition
percentage followed roasted then decaffeinated. The highest concentration of phenolic compounds
and chlorogenic acids were in green coffee followed by roasted, while roasted contained higher
caffeine content than green coffee. Decaffeinated coffee showed considerable amount of total
phenolic and no chlorogenic acids and caffeine were observed.
Conclusions: Coffee exerts variable anticancer effects against OSCC particularly green
coffee.