الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Vitiligo is an acquired skin depigmentation disorder affecting 0.5 -
1% of the world population. The disease may affect individuals of both
sexes. It occurs when melanocytes die or unable to function and mostly
characterized by loss of melanocytes .Despite much research, the etiology
of vitiligo and melanocyte death is not clear. It is related to both genetic
and non-genetic factors, but there are many theories concerning the cause
such as the autoimmune, autocytotoxic and neural hypothesis.
Granzyme B gene is a protein coding gene that is located at 14q12
and has 5 exons. Its protein product is GZMB that is a serine protease
found in the cytoplasmic granules of cytotoxic lymphocytes and NK cells
and plays an important role in inducing apoptotic changes in target cells
during granule exocytosis-induced cytotoxicity.
The aim of this study was to study the role of GZMB gene
polymorphism (rs8192917) in NSV and to correlate the results with the
available clinical data.
This study was carried out at Dermatology and Medical
Biochemistry Departments, Menoufia Faculty of Medicine. It included 40
patients with NSV during the period from June 2019 to October 2019. In
addition to 40 age and sex matched healthy individuals, served as control
group.
All studied patients were subjected to complete history taking as
personal history, present history, family history, history of drug intake,
history of other dermatological autoimmune diseases, history of systemic diseases and thorough general and dermatological examination. VASI score was assessed. Every patient and control subject underwent Laboratory investigations for detection of GZMB gene polymorphism by real-time
PCR.