الفهرس | Only 14 pages are availabe for public view |
Abstract Pregnancy loss is multifactorial involving clinical and biological risk factors. Evidence addressed the association of inherited thrombophilia with pregnancy loss in primigravida with missed abortion in late first triemester. However, the relation between thrombophilia associated gene mutations and adverse obstetric outcome is controversial and data in the literature are inconsistent. The aim of the present study was to investigate the relation of thrombophilia gene mutations, namely, factor V Leiden, and methylene tetra hydrofolate reductase C667T in with primigravida with missed abortion in late first trimester of pregnancy. This cases series descriptive study was conducted on 40 pregnant women with missed abortion in late first trimester from 7week ± 1day to 13 week and 6 day with missed abortion e fetal pole 7 week ± 1day or more in the age between 18 year and 35 year. Blood samples were collected from all pregnant women enrolled in the study for DNA extraction and genotype analysis based on polymerase chain reaction and reverse hybridization. The assay covered two mutations, factor V Leiden, and MTHFR C667T. 52.5 % of cases had MTHFR C667T mutations, with homozygousity in 15 % of cases and heterozygousity in 37.5 % of cases. However, and the 30% of cases had factor V Leiden gene mutations and all are heterozygous. There was a prevalence of 4 cases with combined thrombophilia (Factor V Leiden and MTHFR C677T) in the participating cases. Finally, the number of homozygous and heterozygous individuals were assessed for each of the gene mutations studied. No homozygosity was detected factor V Leiden gene mutation cases |