الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
 |  | from | 150 |  |  |
| | | from | 150 | | |
Abstract
Osteoarthritis (OA) is considered as the most frequent joint disorder, especially in women. It is one of the world’s top ten causes of disability. Knee OA is classified into primary or idiopathic, and secondary. Idiopathic OA is the commonest type of arthritis, it occurs insidiously with advancing age with no obvious predisposing cause. In secondary OA, the predisposing factor can be instability of joints (e.g. meniscus lesions), joint infections, or overload causing repeated excessive wear (marked obesity, work, sports).
OA is a degenerative joint disease resulting from loss of articular cartilage integrity, alterations in the subchondral bone, and inflammation of the synovial membrane. No treatment exists for complete cure of knee OA. Due to the restricted life span of joint arthroplasty, the need for disease-modifying therapies or drugs is high. The ideal treatment for OA should address the mechanical strain that generates joint injury as well as the inflammatory reactions which exacerbates the structural damage.
Intra-articular injection of corticosteroid (CS) has been considered effective in pain relief in cases of knee OA flare. Platelet-rich plasma (PRP) is a biological therapy that has emerged in recent years as an intra-articular injection for long term reduction of pain in knee OA.
The purpose of the current work was to shed light on the possible effects following intra-articular injection of corticosteroid and PRP for treatment of knee osteoarthritis in a rat model of osteoarthritis.
Ten male rats were used for blood collection for preparation of PRP. Then, the present study was carried out on 70 adult female albino, their body weights of average weight 200 gr. The animals were randomly divided into:
group I: (control groups) 30 rats serving as a control group were further subdivided into five equal subgroups 6 animals each:
o Subgroup Ia (control group): received standard diet and free access to water without exposure to any procedure.
o Subgroup Ib (negative control group): received a single knee intra-articular injection by 25 μl of saline.
o Subgroups Ic, Id and Ie (positive control groups): received a single knee intra-articular injection of 0.1 mg of CS (Epirelefan vial containing 40mg/1ml TACA), 50 μl of PRP of protocol 1, and 50 μl of PRP of protocol 2 respectively.
group II: (OA induced untreatd group) 10 rats received a single knee intra-articular injection of 1 mg of MIA dissolved in 25 μl of saline.
group III: (treated group) 30 rats received intra-articular injections of MIA with either corticosteroid or PRP 7 days after intra-articular injections of MIA.
o Subgroup IIIa: (CS treated group) 10 rats received a single knee intra-articular injection of 0.1 mg of CS (Epirelefan vial containing 40mg/1ml TACA).
o Subgroup IIIb: (PRP1 treated group) 10 rats received a single knee intra-articular injection of 50 μl of PRP of protocol 1.
o Subgroup IIIc: (PRP2 treated group) 10 rats received a single knee intra-articular injection of 50 μl of PRP of protocol 2.