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Abstract The main objective of this study is to maximize the utilization of waste paper material and decrease its harmful effect. A total of 11 fungal, 36 bacterial and 39 actinobacterial isolates having cellulase producing ability were selected and tested to assess the cellulose-hydrolysis ability, the findings of this study included the selection of two fungal isolates (A13 and T 20), bacterial isolate B9 and actinobacterial isolate A47. The mesophilic fungal isolates A13 and T 20 were identified as Aspergillus niger strain A13 and Tricoderma sp respectively. The mesophilic actinobacterial isolate A47 and thermophilic bacterial isolate B9 were identified as, Bacillus subtilis ZH strain B9 and Streptomyces violaceolatus ZH strain A47. Fermentation conditions of the aforementioned strains were optimized under both of submerged and solid state fermentation . The hydrolysis of waste material was also investigated, results recorded that all tested strains were able to degrade waste papers to reducing sugar, Trichoderma sp. produced the highest amount of glucose by hydrolysis untreated rice straw. In addition, all tested bio-processed materials exhibited the efficacy to control Fusarium oxisporium as well as Rhizoctonia solani infecting cucumber plants. |