الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Furcation defects are a frequent finding in periodontitis patients. Molars affected with furcation involvement respond less favorably to periodontal therapy, they also exhibit a greater risk for further attachment loss than other teeth.
This study aimed to histologically evaluate guided tissue regeneration procedure using ozone activated platelet rich plasma gel in the management of surgically created critical sized grade II furcation defects.
A split mouth design was carried out using six healthy mongrel dogs. A total of eighteen critical sized grade II furcation defects were surgically created in the third and fourth mandibular premolars, bilaterally. After reflecting a mucoperiosteal flap, the defects in the experimental side were treated with PRP activated by ozone, β-TCP and collagen membranes, whereas, the control side defects were treated with PRP, β-TCP and collagen membrane. The dogs were sacrificed after 4 and 8 weeks. Samples were dissected and prepared for histological evaluation.
Histomorphometric analyses was made on the histological sections in order to calculate percentage of new bone formation in the defect, thickness of new bone trabeculae and the height of the defect fill.
Histological evaluation showed newly forming bone trabeculae of moderate density spanning the whole width between the walls of the mesial and distal roots of the operated tooth and the bifurcation area coronally at one month on the study group, while the control group showed less amount of formed bone, with persistence of greater amount of the scaffold material and less vascularity among the formed bone.
At two months, the study group exhibited an outstanding histological picture; noticeably, more bone formation could be seen in the different regions of the defect, centrally and peripherally where it formed the lateral walls adjacent to the PDL, and at the base of the defect. This was accompanied with excessive cementum deposition on the roots’ surfaces facing the PDL. On the other hand, bone formation and defect fill in the control group was less than its appearance in the experimental group of the same observation period, but it appeared to differ than its appearance in the same group at the previous observation period, showing more bone formation.
The current study concluded that activation of PRP by ozone together with guided tissue regeneration procedures yields favorable effects of accelerating bone formation and vascularization.