الفهرس 
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Abstract
This study was conducted to evaluate the effect of 10% and 1% concentrations of platelet rich fibrin (PRF) exudates obtained from controlled type 2 diabetic patients on the proliferation and osteodifferentiation potentials of dental pulp stem cells (DPSCs) respectively. Materials & methods: The direct method was used to prepare PRF exudates obtained from 8 healthy donors (control group) and 8 controlled type 2 diabetic patients (study group). DPSCs harvested from pulp tissue of impacted lower third molar were treated with 10% PRF exudate obtained from healthy donors and controlled type 2 diabetic patients in 96-well plates for 1,3 and 5 days. The effects of exudates on proliferation and cell viability were tested using the MTS assay. The osteogenic capability of DPSCs was tested using an osteogenic medium for 1 week using concentrations of 1%PRF exudate obtained from healthy donors and controlled type 2 diabetic patients and the osteodifferentiation assay was carried out by alizarin red stain with ELIZA reader. Results: 10% PRF exudate obtained from controlled type 2 diabetic patients had proliferative effect on DPSCs comparable to that of the healthy donors. Similarly 1% PRF exudate obtained from controlled type 2 diabetic patients had osteodifferentiative effect on DPSCs comparable to that of the healthy donors. Conclusion: Based on our study, it is possible to use PRF exudates derived from controlled type 2 diabetic patients to induce proliferation and osteodifferentiation of DPSCs with the recommended concentrations similar to healthy individuals.