الفهرس 
ContentsOnly 14 pages are availabe for public view
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Abstract
Introduction : Decellularization is the process of eliminating the graft cells and genetic materials to develop a personalized scaffold that can be recellularized by person’s own cells. This study was conducted to evaluate the healing capacity of concentrated growth factor (CGF) with or without dental pulp stem cells (DPSCs) seeded on decellularized teeth sections as a biological scaffold for surgically induced calvarial bone defects in albino rats. Materials and Methods: Fifty healthy male albino rats were used in the study. Rats were anesthetized and bilateral calvarial bone defects without including the perichondrium were created, group I (negative control) the calvarial defects were left to heal spontaneously. group II (positive control) the calvarial defects received decellularized teeth section (DTS) only. group III the calvarial defects received CGF seeded on DTS. group IV the calvarial defects were engrafted with 106 DPSCs seeded on DTS, group V the calvarial defects received DTS with CGF and 106 DPSCs. The scaffolds were incubated with either DPSCs, CGF or together for 2 days. The rats were euthanized after 16 weeks, rats’ skulls were surgically removed and processed for histological and immunohistochemical staining with anti TNF-α and anti-osteocalcin followed by histomorphometric and statistical analysis. Results : group I revealed low amount of bone defect healing and statistically significant higher inflammatory response to TNf-α antibody and also gave minimal immune reactivity to osteocalcin antibody. While group II, III, and IV revealed greater amount of regenerated bone trabeculae and revealed variable positive reactivity to TNf-α marker. group V revealed marked enhancement in bone healing and minimal inflammatory response when compared to other groups and also showed highest positive immune reactivity to osteocalcin antibody, while group II, III and IV revealed moderately expressed this marker which confirmed that combination of DPSCs with DTS and CGF having the best impact on enhancing bone formation and reducing the inflammatory reaction during bone regeneration. Conclusions: The results demonstrated that the biological scaffold has a potential impact on calvarial bone healing and suggested enhanced action of DPSCs and CGF in regeneration of bone when they were used locally to treat surgically-induced calvarial bone defects in albino rats with better results were seen with DPSCs more than CGF. However, combination between both CGF and DPSCs gave the best results and their usage may be a novel and effective therapy for treatment of craniofacial bone defects that needs to be further investigated in human.