الفهرس 
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Abstract
The objective of this study is to assess the clinical value of using PRP for management and healing of cut new wounds in donkeys after its preparation from donkey’s whole blood, in addition to compare the efficacy of PRP versus propolis on wound healing.
Sixteen apparently clinically healthy adult male donkeys, aged 1-3 years with body weight ranging from 120-150 kg, were used in this study. Platelets plasma was used after its preparation from the whole blood from each donkey themselves (autologous). It was separated into platelet-rich plasma (PRP), and platelet-poor plasma (PPP). Propolis was used in form of propolis powder after collection from bee hives, dried, grinned and purified from debris. Wound creation was done in back region of animal after being routinely prepared for surgery. Bleeding was controlled by pressure on the wound surface. Animals were kept under observation throughout the period of the experiment (for one month starting from applying of the first dressing). The 16 donkeys were divided into 2 equal groups, each of 8 animals. group 1 (n=8), was used to evaluate the effect of PRP and PPP on wound healing in relation to the control wound. Meanwhile, group 2 (n=8), was used to evaluate the effect of propolis alone or combined with either PPP or PRP. Changing of the dressing has occurred by removing the bandage and adding the dressing materiels, at first, each two days for the first week, then twice weekly for another week, and then once weekly till the end of the first month or complete healing is achieved. 2 ml of plasma either PRP or PPP was injected in the center of the right wound bed and into outward edges of the quadrant of wound lesion. Dressing with propolis powder was carried out by direct pouring of the propolis powder on the wound area till form a thin layer of the powder covering the wound surface. Evaluation of wound healing from beginning of the dressing till end of the experiment or achieving healing based on; clinical wound characters (macroscopic findings), wound measurements and contraction including; measuring mean wound dimensions (length and width), mean wound area (unhealed area) and mean healing percent as well as histologic findings.
Along the course of wound treatment during this investigation, clinical wound parameters including inflammatory signs, exudation, bleeding, infection and granulation tissue formation showed low significant difference between the drugs. These parameters improved with time. Wounds treated with PRP and propolis provided good healing parameters, characterized by absence of inflammatory signs, exudation and infection. Granulation and epithelial tissue formation were moderate to complete and rapidly seen after PRP combined with propolis, especially between 14 and 21 days. Wound contraction showed significant difference among different treatments. There was variation in mean reduction of wound width and length. Wound healing % proved higher following PRP/propolis combination compared with either propolis or PRP alone. The observations concerning histological parameters involved in wound healing allowed the detection of little differences between the groups, overall favoring the group treated with combination of wound dressing. Wounds treated with PRP and Propolis showed covering epithelial cells proliferate and ’crawl’ atop the wound bed, providing cover for the new tissue before completing the second week. Complete re-epithelialization with underlying neovascularization and considerable number of inflammatory cells mainly neutrophil and few fibroplasia were noticed after the first week. After 21 days, complete epithelization with rete ridges formation with underlying granulation tissue formation featuring fibroblast and collagen perpendicular to new blood vessel and parallel to wound surface. Following PRP/propolis combination, complete epithelization with rete ridges with underlying granulation tissue formation featuring fibroblast and collagen perpendicular to few new blood vessel and parallel to wound were observed earlier, after 14 days. Later on, after 21 days, complete scab formation proved clear.