الفهرس 
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Abstract
A laboratory experiment was carried out at Agronomy Department Laboratory of Seed Testing, Faculty of Agriculture, Mansoura University, Egypt, during November and December 2018. The objective of this investigation was to study the effect of methods of application and seeds treatments with some biostimulant substances on improve germination and seedlings vigor of bread wheat Misr 2cultivar. The experiment was conducted in factorial experiment in Completely Randomized Design (CRD) with four replications. The experiment included two factors. The first factor included two methods of application with some biostimulant substances as follows: 1- Soaking seeds. 2- Priming seeds. The second factor integrated with eleven wheat seeds treatments with biostimulant substances as follows: 1- Without treatment (control treatment). 2- Treated wheat seeds with distilled water. 3- Treated wheat seeds with ascorbic acid (AsA) at the rate of 200 mg/L. 4- Treated wheat seeds with salicylic acid (SA) at the rate of 200 mg/L. 5- Treated wheat seeds with humic acid (HA) at the rate of 5 ml/L. 6- Treated wheat seeds with amino acids (AA) at the rate of 50 ml/L. 7- Treated wheat seeds with yeast extract (YE) at the rate of 100 ml/L. 8- Treated wheat seeds with algas extract (AE) at the rate of 2000 mg/L. 9- Treated wheat seeds with the mixture of ascorbic acid (AsA) and salicylic acid (SA) at the same rates. 10- Treated wheat seeds with the mixture of amino acids (AA),yeast extract (YE),algas extract (AE) at the same rates. 11- Treated wheat seeds with all the previous studied biostimulants at the same rates. DATA RECORDED: A. GERMINATION PARAMETERS: 1. Final germination percentage (FGP%). 2. Abnormal seedlings percentage (AS %). 3. Sold seeds (%). 4. Rotten seeds (%). 5. Speed germination index (SGI). 6. Germination index (GI %). 7. Co-efficient of germination (CG). 8. Mean germination time (day). 9. Germination energy percentage (GE %). B. SEEDLING PARAMETERS: 1. Shoot length (cm). 2. Root length (cm). 3. Seedling vigor index (SVI). 4. Membrane permeability percentage (MP %). 5. Shoot fresh weight (g). 6. Root fresh weight (g). 7. Shoot dry weight (g). 8. Root dry weight (g). 9. Photosynthetic pigments. 9.1. Chlorophyll-a . 9.2. Chlorophyll-b. 9.3. Total chlorophylls. 10. Ultra structure of wheat leaf. The most important results obtained from this investigation can be summarized as follows: A. Effect of application methods: The obtained results of this study showed that methods of application with some biostimulant substances (soaking and priming seeds) significantly affected germination parameters (final germination, abnormal seedlings, sold seeds and rotten seeds percentages, speed germination index, germination index, co-efficient of germination, mean germination time and germination energy percentage) and seedling parameters (shoot length, root length, seedling vigor index, membrane permeability percentage, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight and photosynthetic pigments likes; chlorophyll-a, chlorophyll-b and total chlorophylls). It could be noticed that priming method of seeds wheat with biostimulant substances surpasses soaking method in some germination parameters (final germination percentage, germination index, and co-efficient of germination) and some seedling parameters (shoot length, seedling vigor index, membrane permeability percentage, shoot fresh weight, root fresh weight, shoot dry weight and photosynthetic pigments likes chlorophyll-a, chlorophyll-b and total chlorophylls). Nevertheless, soaking method with biostimulant substances surpasses priming method in some germination parameters (abnormal seedlings, sold and rotten seeds percentages, speed germination index, co-efficient, mean germination time and germination energy percentage) and some seedling parameters (root length and root dry weight). B. Effect of seeds treatments with biostimulants: Seeds treatments with some biostimulant substances (ascorbic acid, salicylic acid, humic acid, amino acid, yeast extract and alga extract and some mixture of them) significantly affected germination parameters (final germination, abnormal seedlings, sold seeds and rotten seeds percentages, speed germination index, germination index, co-efficient of germination, mean germination time and germination energy percentage) and seedling parameters (shoot length, root length, seedling vigor index, membrane permeability percentage, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight and photosynthetic pigments likes; chlorophyll-a, chlorophyll-b and total chlorophylls). from obtained results, it could be observed that treating wheat seeds with yeast extract treatment surpasses the other studied seeds treatments with biostimulants in speed germination index, mean germination time, germination energy percentage, root fresh weight and root dry weight. On the other hand, it could be noticed that treating wheat seeds with the mixture of ascorbic acid and salicylic acid treatment surpasses other studied seeds treatments with biostimulants in final germination percentage, germination index, shoot length, root length, seedling vigor index. It also recorded the lowest percentage of abnormal seedlings. However, treating wheat seeds with the mixture of humic acid + amino acid + algea extract + yeast extract surpasses other studied seeds treatments with biostimulants chlorophyll-a, chlorophyll-b, and total chlorophylls. In addition, treating wheat seeds with amino acid was the best treatment in germination index, shoot fresh weight, and shoot dry weight. Nevertheless, treating wheat seeds with the mixture of ascorbic acid + salicylic acid + humic acid + amino acid +yeast extract + algae extract treatment was the best in rotten seeds percentage it was recorded the lowest percentage. On the other hand, treating wheat seeds with the distilled water was the best in membrane permeability percentage. As we can see, treating wheat seeds with amino acid and yeast extract was the lowest in sold seeds percentage, and treating wheat seeds with algae extract was the best in Co-efficient of germination. C. Effect of the interaction between application methods and seeds treatments with bio-stimulants: The achieved results of this study showed that the interaction between application methods and seeds treatments with some biostimulant substances significantly affected germination parameters (final germination, abnormal seedlings, sold seeds and rotten seeds percentages, speed germination index, germination index, co-efficient of germination, mean germination time and germination energy percentage) and seedling parameters (shoot length, root length, seedling vigor index, membrane permeability percentage, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight and photosynthetic pigments likes; chlorophyll-a, chlorophyll-b and total chlorophylls). It could be noticed that soaking wheat seeds in yeast extract surpasses other studied treatments and resulted in the highest values of speed germination index, mean germination time, germination energy percentage and shoot dry weight. While, the highest value of root dry weight was obtained when priming seeds in amino acid. Priming wheat seeds with amino acid was the best treatment for final germination percentage, germination index, germination energy percentage, shoot fresh weight, which amino acid resulted in the highest values of these traits. However, priming wheat seeds with the mixture of humic acid + amino acid + algea extract + yeast extract surpasses other studied treatments in abnormal seedlings percentage, chlorophyll-a, chlorophyll-b and total chlorophylls contents in shoot. On the other hand, priming wheat seeds with the mixture of ascorbic acid and salicylic acid treatment surpasses other studied treatments in shoot length, root length. Whereas, the highest value of root dry weight was produced from soaking wheat seeds with the mixture of ascorbic acid and salicylic acid. As we can see, soaking wheat seeds in amino acid and yeast extract gave the lowest values of sold seeds percentage on the other hand, soaking wheat seeds in ascorbic acid gives the highest values of mean germination time. However, soaking wheat seeds in humic acid registered the highest co-efficient of germination. As we can see priming wheat seeds in without treatment (control treatment) and mixture of ascorbic acid + salicylic acid + humic acid + amino acid +yeast extract + algae extract treatment registered the lowest percentage in rotten seeds. In addition, soaking wheat seeds in mixture of all treatments surpasses other treatments in membrane permeability percentage. Moreover, soaking or priming wheat seeds in algea extract was the best in co-efficient of germination. Priming wheat seeds in salicylic acid gave the highest seedling vigor index. CONCLUSION from obtained results of this study, it could be priming seeds of bread wheat Misr 2 cultivar with the mixture of ascorbic acid and salicylic acid at the rate of 200 mg/L of each or yeast extract at the rate of 100 ml/L to improve germination and seedling vigor.