الفهرس 
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Abstract
The present investigation was designed to study the quality of of 200 random samples of fish stored in ice ( 50 each of mullet fish , Nile tilapia , mullus surmuletus ( Barboni ) and sparus aurata ( Denise ) were randomly collected from different fish markets at El Behira Province .the experiment relaying on sensory assessment (odour, color and consistency) , and microbiological assessment(total bacterial count, total coliform count, total psychtrophic count, Enterobacteriacea count, detection of vibrio parahaemolyticus and detection of salmonella).
The results revealed that:
1. All fish samples examined through odour, color and consistency founded normal and apparently normal.
2. Total bacterial count:
the total bacterial count of the examined fishes cfu/g ranged from 0.8 x 103 to 7x106 with an average of 1.62 x 106 ± 0.65 x 106 for barboni, 2.5 x 104 to 5.5 x 106 with an average of 1.48 x 106 ± 0.44 x 106 for Denise, 2.5 x 104 to 7 x 107 with an average of 9.99 x 106 ± 3.49 x 106 for mullet and 1 x 104 to 5 x 107 with an average of 1.13 x 107 ± 0.38 x 107 for Nile tilapia. Means denoted within the same column with different superscripts differ significantly at (P<0.05).
Comparing the obtained results with E.O.S (889-1/2005) which stated that the permissible limits of total bacterial count is not exceed 106, it revealed that 76% of samples accepted and 24% un-accepted for barboni, 72% of samples accepted and 28% un-accepted for Denise, 48% of samples accepted and 52% un-accepted for mullet and 44% of samples accepted and 56% un-accepted for Nile tilapia.
3. Total coliform count:
The total coliform count of all examined samples of barboni, Denise, mullet and Nile tilapia were contaminated with coliforms bacteria. Furthermore, total coliforms count in the examined samples of examined fishes ranged from 3 x 104 to 4 x 106 with a mean value of 1.17 x 106 ± 0.34 x 106 cfu/g for barboni, 6 x 104 to 8.5 x 106 with a mean value of 2.21 x 106 ± 0.68 x 106 cfu/g for Denise, 3 x 104 to 2 x 107 with a mean value of 4.19 x 106 ± 1.52 x 106 cfu/g for mullet and 2.5 x 104 to 4 x 107 with a mean value of 6.17 x 106 ± 2.65 x 106 cfu/g for Nile tilapia.
Means denoted within the same column with different superscripts differ significantly at (P<0.05).
4. Total psychtrophic count:
The total psychtrophic count of all examined fishes sample ranged from 0.3 x 105 to 6 x 106 with a mean value of 1.71 x 106 ± 5.45 x 105 cfu/g for barboni, 0.2 x 105 to 7 x 105 with a mean value of 2.45 x 105 ± 0.63 x 105 cfu/g for Denise, 0.2 x 105 to 5 x 106 with a mean value of 1.58 x 106 ± 4.7 x 105 cfu/g for mullet and 0.4 x 105 to 5.5 x 107 with a mean value of 9.07 x 106 ± 3.59 x 106 cfu/g for Nile tilapia.
Means denoted within the same column with different superscripts differ significantly at (P<0.05).
5. Enterobacteriaceae count:
The total enterobacteriaceae counts of the examined samples of fish varied from 2 x 104 to 7 x 106 with an average of 1.78 x 106 ± 5.98 x 105 cfu/g for barboni, 3.5 x 104 to 6 x 106 with an average of 1.79 x 106 ± 5.23 x 105 cfu/g for Denise, 2.5 x 104 to 1 x 108 with an average of 1.59 x 107 ± 5.73 x 106 cfu/g for mullet and 2 x 104 to 2.5 x 107 with an average of 6.37 x 106 ± 2.17 x 106 cfu/g for Nile tilapia.
Means denoted within the same column with different superscripts differ significantly at (P<0.05).
6. Detection of vibrio parahaemolyticus:
Detection of Vibrio parahaemolyticus in examined fish samples revealed that 28%, 16% and 14% of the examined samples barbone, Denise and Mullet were contaminated with V. parahaemolyticus, respectively. In contrast, all examined samples of Nile tilapia were free from such organisms. Comparing these results with E.O.S (2005) the results of barboni, Denise and mullet are not appropriate with Egyptian organization for standardization and quality control for the examined samples as it should be free. But Nile tilapia is compatible and free from vibrio parahaemolyticus.
7. Detection of salmonella:
Detection of Salmonella in examined fish samples revealed that Salmonella organisms were recovered from 10%, 6% and 8% of the examined samples of barboni, Mullet and Nile tilapia. In contrast, all examined samples of Denise were free from Salmonella. This result of barboni, Mullet and Nile tilapia was not in agreement with the Egyptian standards (E.S/2009) of marine fish that reported that all fish sample must be free from salmonellosis in 25g.
The serotypes of Salmonella organisms isolated from examined samples of barboni, Mullet and Nile tilabia were Sal. enteritidis (4%, 4% and 4%) and Sal. typhimurium (4%, 2% and 4%), respectively. However, only 1 strains 2% isolated from barboni were serologically identified as Sal. haifa.
The public health significance of contaminants and the possible sources of contamination of farm fishes with these organisms as well as suggestive hygienic measures to increase the quality of such fishes were discussed.