الفهرس 
Introduction and aim of the workOnly 14 pages are availabe for public view
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Abstract
Summary
chronic hepatitis caused by HCV often leads to fibrosis and cirrhosis, which markedly increase the risk of developing HCC.
Around 15% of chronically infected HCV patients develop to liver cirrhosis and finally, 5% of these liver cirrhosis patients might develop to HCC through five years .
Egypt has one of the highest prevalence rates of HCV in the world with about 10% chronic HCV infection among people aged 15 to 59 years.
It is well established that the outcome of HCV infection is some what influenced by host factors such as advanced age, male gender, obesity and host genetics .
Based on this, there is a growing interest in the identification of host genetic variations that may also play a role in the host response to infection and the clinical outcome of the disease .
Genotyping of DEPDC5 rs1012068 T/G gene polymorphism was used to assess its frequency as genetic susceptibility factors for end-stage liver disease progression in HCV.
This study was conducted to assess the association of DEPDC5 rs1012068 T/G gene polymorphism among chronic HCV-infected patients with disease progression and chronic HCV related complications as regards fibrosis, cirrhosis, and HCC.
The study was conducted on 120 subjects divided into 2 groups:
Patients group:
Included 60 chronic hepatitis C virus patients with age range from 40-64 years. Those patients were recruited from those attending Outpatients Clinic and Tropical Medicine Inpatient Department, Benisuef University Hospital. Patients group was classified into 3 subgroups:
group I (Fibrotic group): Twenty patients with liver fibrosis.
group II (Cirrhotic group): Twenty patients with liver cirrhosis.
group III (HCC group): Twenty cirrhotic patients with hepatocellular carcinoma (HCC).
Control group:
Included 60 apparantly healthy volunteers with age range from 48-62 years who were age matched with patients group.
Patients and healthy controls were recruited from Beni-suef University Hospital after informing the participants about the study, taking their consent and the approval of the ethical committee was be taken.
• All subjects were subjected to the following laboratory investigations:
• Routine Laboratory Investigations: Hepatitis C virus antibodies (HCV Abs), Hepatitis C virus RNA by polymerase chain reaction (PCR), Complete Blood Count (CBC), Alanine transaminase (ALT), Aspartate transaminase (AST), Albumin, Bilirubin ,Prothrombin time, Prothrombin concentration and international normalized ratio( PT , PC and INR), Alpha-feto protein.
• Specific Laboratory Investigations: Study of the DEPDC5 rs1012068 T/G gene polymorphism by Real-time PCR TaqMan allelic discrimination assays.
Results of the current study revealed that:
In our study, we found that mutant G allele of DEPDC5 rs1012068 T/G gene showed statistically significant higher frequency among fibrosis group versus control group, Cirrhosis group and HCC group (p= 0.011, p= 0.115 and p= 0.012, respectively). Also, mutant G allele showed statistically significant higher frequency among both fibrosis and cirrhosis groups versus HCC group (p= 0.046) and fibrosis group versus both HCC and cirrhosis groups (p= 0.016).