الفهرس 
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Abstract
Primates are one of the animal species closely related to humans but they may nevertheless be considered as a main source of diseases for both animals and humans .
The current study was conducted throughout 2o18/2o19 at the Alexandria Zoological garden, Egypt to investigate the presence of E.coli in apparently healthy zoo primates in the Alexandria zoo . Studying its virulence and resist it by diagnosis and treatment.
The samples were examined bacteriologically by direct and indirect methods to determine different virulence genes appear in primates , and isolates were confirmed biochemically.
The obtained results were summarized as following :
1- The incidence of E. coli bacteria in apparently healthy primates in Alexandria zoo, Egypt . Isolation and identification E. coli bacteria .
2- Out of fifty four examined zoo primates were recovered. apparently healthy of different ages, sex and different breeds (Hamadryas baboons , Olive baboon ,Yellow baboon, Stump tail macaque , Bonnet macaque , Rhesus macaque , Grivet monkey , Patas monkey and Tufted capuchin monkey) , bacterial isolation was carried out by means of rectal swabs , It was found that 44 samples were positive for E. coli with percentage of 82% .
3- Identification of bacterial isolates was carried out and clarified the presence of serotypes; O158H4, O158H2, O158H5, O158H20,O78H4, O18H2, O18H6, O114H2 and O78H6
4. Sixteen isolates of E. coli were subjected to PCR examination . It was found that all of 16 isolates of E. coli were positive for genes phoA, Iss and iutA while they were negative for eaeA virulence gene.
5- The antibacterial sensitivity test cleared that Enrofloxacine, Colistin, Ciprofloxacin, Cefotaxime, Gentamicin and Cefifur were antibiotics of choice to deal with E. coli in Alexandria zoo primates.
The results demonstrated that primates may carry and spread these bacteria so, preventive measures for human exposure should be followed as these microorganisms are of public health concerns.