الفهرس 
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Abstract
This present study aimed detect the expression of microRNA-520 in psoriatic skin of diseased patients as compared to normal control persons to investigate the possible role of microRNA-520 in the pathogenesis of this disease.
It was a case-control study included 30 psoriasis patients from both sexes. They all presented to dermatology department at Beni-Suef University hospital. The psoriasis patients were 19 males and 11 females’ patients, their age ranged from 18 to 75 years, the average age was, (46.13±13.8). And 30 healthy controls were taken, they were age and sex matched to the psoriasis cases.
All the sixty individuals included in the study was subjected to a written informed consent for participation in the study, detailed history taking, clinical assessment to determine type, onset, course, family history and disease duration of Psoriasis. Then all participants underwent blood samples collection in BD Vacutainer K2EDTA tubes using a 21-gauge needle. Then centrifuged at 4000 rpm for 10 min at 4°C, plasma separated, and stored at −70°C. RNA Extraction from the plasma using the miRNeasy extraction kit according to the manufacturer’s instructions.
The collected data were coded then entered and analyzed taking three months using the SPSS version 22.
The analysis of microRNA-520 expression values in the serum of our studied population (patients with psoriasis lesion & control skin) reveled that, expression of (MicroRNA-520) was significantly lower in psoriasis patients as compared with healthy controls; the mean expression values were (0.342 vs. 1.015) in psoriasis cases and healthy controls respectively with a statistically significant p-value< 0.001.
Expression of MicroRNA-520 was significantly lower in male psoriasis patients as compared with females; (p-value= 0.032). In the current study we found no detected relation between expression of MicroRNA-520 with disease onset, course, duration, family history, PASI score and patients’ age, unfortunately, we did not find similar studies to compare the results obtained from our current study.
MicroRNA-520 could play important role in psoriasis pathogenesis and it may be a novel target for the treatment of psoriasis.