الفهرس 
cover eOnly 14 pages are availabe for public view
 |  | from | 114 |  |  |
| | | from | 114 | | |
Abstract
Purpose and aim of the work: To compare between Femtoseconds laser-assisted keratoplasty FAK) and penetrating keratoplasty (PK) using microscopic examination (light and transmission electron microscopy) of the cut edges of the remaining donor cornea. Material and Methods: Prospective observational study involving 10 eyes; 5 eyes by femto assisted keratoplasty FAK (top hat technique) and the other 5 eyes by conventional trephination method. Main outcomes are histological findings at the cut edge of the donor corneal graft tissue (at 3, 6, 9, and 12 o’clock). Results: In femto assisted trephined corneas (FAK), cellular and ultra-cellular changes in the form of marked edema, disruption and disorganization of the collagen fibers, and nuclear changes were more pronounced as compared to conventional PK. In addition, a band of debris with disrupted collagen fibers extending from the anterior to posterior stroma was evident in the corneal stromal layer, compared to very little stromal edema with mechanical trephination. Conclusion: The electro microscopic evaluation of the cut donor edges showed less microstructural damage of corneal tissue in the conventional PK compared to FAK. Further studies are required to evaluate the FS induced in-vivo ultrastructural changes and correlate changes in the corneal tissue status with the clinical outcomes at the levels of healing, and biomechanical stability.