الفهرس 
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Abstract
Meat is a good source of protein which makes it a good substrate for microbial growth. In this study, the prevalence, antibiogram and virulence genes of some foodborne pathogens from hospital beef meals provided to intensive care patients were evaluated. Therefore, a total of 60 beef meat samples (30 raw and 30 cooked samples) were randomly collected from different hospital kitchens at Ismailia province, Egypt. Samples were subjected to bacteriological evaluation for Staphylococcus aureus, Escherichia coli and Salmonella. The results of the current study revealed that S. aureus, E. coli and Salmonella were isolated by a prevalence of 38.3% (23/60), 16.6% (10/60), and 8.3% (5/60), respectively. The antimicrobial sensitivity testing for the isolated was determined. S. aureus were harbored sea, seb, sec and sed enterotoxin genes with a prevalence of 13.04%, 4.34%, 13.04 % and 4.34%, respectively. E. coli isolates were harbored stx1 and stx2 with a prevalence of 83.33% and 66.66%, respectively. E. coli was serotyped as O128, O142, O26 and O157. Salmonellaharbored invA and hilA genes by 100% and 80%, respectively. In conclusion, application of good manufactures practices with well-trained food handlers are necessary to control and prevent contamination of the hospitable meals by foodborne bacteria in order to protect and safe the intensive care patients.