الفهرس 
صفحة العنوانOnly 14 pages are availabe for public view
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Abstract
Abstract
Lutein is a potent antioxidant that is found in ocular tissue. It protects retina against oxidative stress. At the same time cisplatin is one of common chemotherapy which is used to treat many types of cancer. This study aimed to increase lutein efficiency by encapsulating it into liposome and testing its Neuroprotective effect against cisplatin-induced retinal injury in rabbit. Twenty-four male, New Zealand, rabbits weighing 1.5–2 kg were divided into four groups, as follows: group I: as a control. group II: received cisplatin only. group III: received free lutein + cisplatin.
group IV: received liposomal lutein + cisplatin.
All treatments were administrated twice per week for 14 days. Electroretinogram (ERG) was recorded for all rabbits just before decapitation. Then, the retinae were subjected to histopathological evaluations and comet assay.
Results indicated significant decrease (p ˂ 0.01) in ERG waves, significant increase (p ˂ 0.01) in all parameters of comet assay (% tailed cells, tail length, DNA% in tail and tail moment), severe fragmentation in photoreceptors layer and changes in inner retina after the administration of cisplatin. There were some sort of improvement in ERG, comet assay and the histological results after the administration of lutein with cisplatin, whereas these tests yielded values comparable to control in the liposomal lutein group. We found that lutein was replaced by liposomal lutein to raise its efficiency. Liposomal lutein administration exerted a significant effect in preventing the harmful effects of cisplatin on the function of the retina, protecting DNA from damage, and
might also avert the histopathological damage in the rabbits’ retina. Lutein is organic. Therefore, using it as a treatment, using any artificial chemicals was avoided, which might be preferable but with the risk of highly toxic effects. This preclinical study needs more investigations for extensive evaluation of this new technique.