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Abstract Chicken meat is considered as a major source of animal derived protein worldwide. Chicken is regarded as a popular food among children because of its high palatability and digestibility, specific aroma, and flavor. However, it is also a potential source of foodborne pathogens such as E. coli and Salmonella spp., and chemical residues such as biogenic amines. This study aimed at investigation of the prevalence rates of E. coli and Salmonella spp. in the retailed chicken breast in rural areas located in Sharkia Governorate, Egypt. Furthermore, serological identification of the recovered E. coli and Salmonella spp., was followed. Antimicrobial susceptibility testing of the obtained E. coli and Salmonella isolates was examined. PCR screening of drug-resistance coding genes among the identified isolates was tested. Finally, residues of three biogenic amines including HIS, CAD, and PUT were quantitatively estimated using HPLC. |