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العنوان
Prevalence, Resistance profile & Virulence genes of Streptococcus Agalactiae Colonizing Near-Term Pregnant Women Attending
Ain Shams University Hospital
/
المؤلف
Abdallah , Noha Gad
هيئة الاعداد
باحث / نهي جاد عبدالله
مشرف / فاتن مصطفي علي
مشرف / لمياء عبدالمنعم عادل
مشرف / ولاء عبداللطيف ابراهيم
مشرف / أحمد محمد القطب
تاريخ النشر
2021
عدد الصفحات
191 p:
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علم الأحياء الدقيقة (الطبية)
تاريخ الإجازة
1/1/2021
مكان الإجازة
جامعة عين شمس - كلية الطب - الميكروبيولوجيا الطبية والمناعة
الفهرس
Only 14 pages are availabe for public view

from 191

from 191

Abstract

group B streptococcus (GBS) is a common cause of infections in pregnant females and non-pregnant adults with chronic diseases (such as diabetes and cancer), also it is the main reason of septicaemia and meningitis in infants. The aim of this study was to figure out how common GBS is in pregnant women, the antimicrobial sensitivity pattern of the isolated GBS colonies and check the presence of scpB and rib virulence genes in these isolates. We screened 203 pregnant women attending the maternity Hospital of Ain Shams University using vaginal sampling. Isolation was done on chrOMagartM Strep B and sheep blood agar plates then identified via colony characters, Gram stain, test for catalase production, christie–Atkins–Munch-Petersen (CAMP) test, test for hippurate hydrolysis and latex agglutination test. This was followed by an antibiotic susceptibility test. Finally, Detection of scpB and rib virulence genes by conventional PCR was done. Our study detected that the prevalence rate of GBS in involved pregnant women was 11.33%. A statistically significant association between colonization and history of spontaneous abortion and preterm labor was observed. chrOMagar™ Strep B showed the same sensitivity of sheep blood agar with extensive effort to isolate suspected GBS colonies from blood agar. GBS was 100% sensitive to levofloxacin, linezolid, cefepime, ceftaroline and ceftriaxone. Also, it was highly sensitive to vancomycin (91.3%). Sensitivity to clindamycin, azithromycin, penicillin and ampicillin was (21.70%, 21.70%,47.80%, 47.80%) respectively. The least sensitivity of GBS was to erythromycin ( 8.7%). All isolates possessed the scpB gene (100%) while only 18 isolates (78.26%) had the rib gene