الفهرس 
IntrductionOnly 14 pages are availabe for public view
 |  | from | 166 |  |  |
| | | from | 166 | | |
Abstract
The aim of this work is to study the toxic effect of intraperitoneal
administration of gold nanoparticles -if any- on the mucous membrane
and salivary glands of the tongues of albino rats and the possibility of
one-month recovery period.
Forty-six adult male albino rats with an average 150-180 gram body
weight were used in the present investigation. They were divided into the
following groups:
group 1: consisted of 16 rats and served as controls, they received the
solvent of AuNP (0.5ml deionized water) intraperitoneally daily for 21
days.
Half the animals of the control group (sub-group 1.1) was euthanized
after 21 days, while the other half (sub-group 1.2) was left untreated for
one more month, then euthanized.
group 2: consisted of 15 rats. They received 10mg/kg body weight of
AuNP solution
265
dissolved in 0.5ml deionized water (particle sizes
around 30 nm)
266
intraperitoneally daily for 21 days.
group 3: consisted of 15 rats. They were treated the same way as
group 2 animals for 21 days and then left for one-month as recovery
period.
The experiment lasted for 21 days for group 2 then the rats were
euthanized by cervical dislocation. While the rats of group 3 were
euthanized the same way after one month of stopping treatment for
recovery; their tongues were dissected out, fixed in 10% neutral buffered
formalin, processed, and embedded in paraffin. Six microns’ thick
sections were cut to be stained with:
Hematoxylin and eosin for detection of any possible structural
changes in the mucous membrane and salivary glands of the
tongues.
Mouse monoclonal antibody for localization of PCNA to detect
any possible changes in its index.
Representative tongue specimens from the different groups were fixed
in glutaraldehyde (3%), dried in a desiccator under vacuum, gold
sputtered to be examined with the scanning electron microscope to detect
any possible ultrastructural changes in its dorsal surface.
Results:
I. Histological Results: (Haematoxylin and Eosin stain)
The histological examination of the mucous membrane of the
tongue of the control rats showed the normal histological features of
surface epithelium and underlying lamina propria both in sub-groups 1.1
and 1.2.
Filiform papillae appeared sharp conical and the fungiform papillae
appeared rounded, mushroom like with flattened top and a single well
developed taste pore at the middle of the superior surface of each papilla.
Well-formed connective tissue in the lamina propria and muscle fibers
running in different direction. The circumvallate papilla was surrounded
with a circular furrow.
At the bottom of the furrow opened the ducts of pure serous salivary
glands situated beneath the circumvallate. They were formed of normal
serous acini. Pure mucous salivary glands were also seen among the .