الفهرس 
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Abstract
The present thesis includes preparation, characterization and evaluation of the structural changes of the prepared systems. The present study can be divided into two basic sections. Section (I) concern ’’Structural properties and preparation of pure materials (Polyvinyl alcohol and Dye) in which polymer was prepared via casting technique method and phenolic dye was synthesized through diazo-coupling reaction followed by condensation reaction and finally Hantzsch-type cyclization. In this section x-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) were employed to test the physical and chemical characteristics of pure materials, while ultraviolet-visible (UV/Vis.) used to study optical properties, scanning electron microscopy (SEM) for surface nature and Swelling ratio for solvents holding capacity at different pH values. The final test is a very important role in the antibacterial activity and biomedical application. Sections (II) concern ”Testing of structural, thermal and chemical properties as well as the antimicrobial activity of Polyvinyl alcohol matrix modified with different concentrations of phenolic dye” by the same techniques in section (I), in addition to test antimicrobial characterization of blend containing different amounts of dye. So this section represents the important part of this study related to wound healing. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), UV-visible, scanning electron microscopy (SEM) were used to test the physical, chemical and structure but antibacterial activity and Swelling ratios were used to test ability of system in biomedical applications like (restoration and healing wounds). FTIR spectra of PVA host polymer modified with a minor dopant of phenolic dye shows a minor variation in the vibrational bands associated with the presence of O-H group vibration indicating some interaction of phenolic dye with the host matrix via hydrogen bonding, UV/Vis. spectra of PVA and phenolic dye combination verified good information about optical properties, XRD diffractograms verified information about nature (crystal/amorphous) lattice arrangement and reveals compatibility with the FTIR spectrum of PVA blend with phenolic dye, SEM images explained highly incorporation between PVA blend and phenolic dye by studying porosity of the surface. Antibacterial capacity evaluated against two gram positive bacteria (Staphylococcus aureus and Bacillus subtilis), two gram-negative bacteria (Escherichia coli and Pseudomonas aeuroginosa) and anti-fungal activities test against one fungus (Candida albicans) which results shows the studied samples at the ambient room temperature (RT). At 0.001 wt percent, the inhibitory zone was shown to be greater against Escherichia coli and Bacillus subtilis. Against all test cultures, antibacterial activity indices of 43.7% and 41.66% were found (Escherichia coli and Bacillus subtilis, respectively). The activity indices for Staphylococcus aureus and Pseudomonas aeruginosa were 39.47% and 34.28%, respectively. At a concentration of 0.001 weight percent, Candida Fungi had an activity index of 40.62%. At 0.004 wt percent, the inhibitory zone was shown to be greater against Escherichia coli and Staphylococcus aureus, antibacterial activity indices of 78.1% and 78.94% were found (Escherichia coli and Staphylococcus aureus, respectively). The activity indices for Bacillus subtilis and Pseudomonas aeruginosa were 66.6% and 62.85%, respectively. At a concentration of 0.004 weight percent, Candida Fungi had an activity index of 62.5%. Bacillus subtilis was found to be the most sensitive to dye dopant, whereas Escherichia coli was shown to be the least susceptible. The antibacterial activity of phenolic dye was significantly increased when the dopant content of synthesized dye was increased. This might be attributed to a change in the crystallite size and concentration of the phenolic dye.