الفهرس 
Aim of The WorkOnly 14 pages are availabe for public view
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Abstract
Melasma is a common acquired disorder of hyperpigmentation.
The disorder is much more common in women, particularly of
reproductive age, and in darker skin types. Melasma has a deleterious
impact on a patient’s quality of life. Free radical injury has been
suggested as a possible pathogenic mechanism in the causation of
melasma. Moreover, it was reported that anti-oxidants are effective in
melasma treatment.
The study aimed to assess the possible role of glutathione
peroxidase enzyme in Melasma patients through its
immunohistochemical evaluation in involved skin compared to normal
skin.
This case control study was carried out on twenty patients with
melasma and twenty age and gender matched healthy subjects as a
control group. Patients were selected from Dermatology Outpatient
Clinic, Faculty of Medicine, Menoufia University Hospital, during the
period between December 2019 and March 2020. Normal skin
biopsies were taken from subjects attending Plastic Surgery
Department. Written consent form approved by Committee of Human
Rights in Research in Menoufia University was taken from every
participant before study initiation.
All studied cases were submitted to complete history taking,
clinical and dermatological examination including assessment of
mMASI score. Skin biopsies were taken from every case under local
anesthesia from lesional skin and sent to Pathology Department,
Faculty of Medicine Menoufia University for routine tissue processing
and preparation of hematoxylin and eosin (H&E)stained slides and
anti-GPX1 immunohistochemical stained slides.
In this study, there was a significant relationship between
epidermal intensity of GPX1 and the degree of dermal inflammation,
since mild and moderate inflammation was associated with mild
epidermal intensity of GPX1 compared to cases showing severe
inflammation which were associated with moderate epidermal
intensity of GPX1,Also there was a significant relationship between
epidermal GPX1 distribution and the degree of dermal inflammation,
Since mild and moderate inflammation was associated with focal
GPX1 epidermal expression compared to cases showing severe
inflammation which were associated with diffuse epidermal
expression of GPX1.
In addition, there was a significant correlation between
epidermal distribution of GPX1 and severity of melasma assessed by
modified MASI score, since high mean and median score was
associated with diffuse epidermal expression compared to cases
showing focal expression, Also there was a statistically significant
association between epidermal intensity of GPX1 and severity of
melasma assessed by modified MASI score.
The current study demonstrated that GPX1 was expressed
diffusely in epidermis of all control cases most of them of moderate
intensity while in most of melasma cases it is focally expressed with
mild intensity.
These results may have an explanation for the role of GPX1 as a
protective enzyme against oxidative stress which plays a major role in
the development of melasma.