الفهرس 
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Abstract
In vitro fertilization (IVF) has been considered as an effective infertility treatment, oocyte quality is important for the success of IVF, there are many factors affecting oocyte quality including oxidative stress, melatonin has a role in oocyte maturation and protection from oxidative stress infertility definition, infertility causes and treatment, Oxidative stress may lead to female infertility, different oxidative stress markers, melatonin as an antioxidant in the follicle, melatonin can also protect oocytes from ROS during IVM. Follicular fluids were collected from 100 women undergoing IVF-ET at the time of oocyte retrieval. Oxidative stress markers 8-OHdG, MDA and H2O2and the antioxidant melatonin were measured in each sample. Buffalo oocytes were incubated in in vitro maturation media with different concentrations of melatonin in presence of H2O2. Follicular fluid 8-OHdG was significantly higher in the high rates of degenerated oocytes group, 8-OHdG had a significant inverse correlation with melatonin (p=0.00). In vitro maturation of buffalo oocytes in maturation media containing different concentrations of melatonin in presence of H2O2, melatonin improved oocyte maturation depending on the dose. Melatonin level naturally decreases with increasing women ages. 8-OHdG was may be used as a marker in predicting oocyte quality. Melatonin improved the maturation rate of buffalo oocytes in presence of H2O2, it has a protective role against the toxic effects of oxidative stress in vitro.