الفهرس 
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Abstract
Dental esthetic needs are increasing in recent years with a greater demand on pleasant appearance; including a healthy set of dentitions but also esthetically improved gingival appearance, especially when it is located in the anterior labial region. Physiologic gingival hyperpigmentation affects numerous people of different ethnic backgrounds.
Gingival depigmentation is defined as a periodontal plastic surgical procedure that are aimed at removing the pigmented gingiva and methods aimed at masking the pigmented gingiva. Techniques of gingival depigmentation can be classified as; chemical methods mainly by ascorbic acids, surgical methods, electrosurgery, cryosurgery, radiosurgery, and lasers.
The study was conducted on eight patients seeking treatment for their gingival hyperpigmentation for esthetic reasons. A total number of 16 sites of facial gingival hyperpigmentation were treated, two in each patient in a split mouth technique.
• group I (LASER with I-PRF): Included eight sites that were treated with laser technique for gingival depigmentation, followed by intramucosal field injection of I-PRF (as study group).
• group II (LASER): Included eight sites that were treated with laser technique for gingival depigmentation (as a control group).
The clinical outcomes were assessed five time for each patient: at baseline (pre-operative), post-operative, one week, one month and three months post-operatively. Clinical assessment was done by measuring DOPI, clinical wound healing, VAS and patient satisfaction questionnaire.
In group (I), The mean of DOPI decreased from (2.75±0.46) to (0.00±0.00) while in group II it decreased significantly from (2.75±0.46) to (0.00±0.00) from baseline to 1 day, 1 week and 1 month after treatment
For histological assessment biopsies were taken for each patient in one occasion: it was taken from the same side after one week. The slides were stained with H&E stain and captured using digital camera then transferred to the computer system for image analysis of epithelial thickness, inflammatory cell infiltrate
Epithelial thickness of group (I) (853.28±126.70) had a significantly higher mean value than group (II) (460.14±71.02) (p<0.001). and Number of blood vessels in group (I) Mean value (24.62±2.07) was higher than that of group (II) (22.38±4.07) yet the difference was not statistically significant (p=0.234).
Majority of group (I) 5 (62.5%) showed moderate inflammatory cell infiltrate, while in group (II) most of the cases 3(37.5%) were equally divided between having moderate and severe inflammatory cell infiltrate and there was no significant difference between both groups (p=1).