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العنوان
CHEMICAL, BIOLOGICAL AND HISTOPATHOLOGICAL STUDIES ON MATCHA TEA USING EXPERIMENTAL RATS /
المؤلف
Abdelal, Nehad Akram Mohamed Ali.
هيئة الاعداد
باحث / نهاد اكرم محمد على عبدالعال
مشرف / عماد محمد عبد الحليم الخولي
مشرف / طارق محمد عبد الرحمن
الموضوع
nutrition.
تاريخ النشر
2022.
عدد الصفحات
146 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
اقتصاد منزلي
الناشر
تاريخ الإجازة
19/2/2022
مكان الإجازة
جامعة المنوفية - كلية الإقتصاد المنزلى - تخصص التغذية وعموم الأطعمة
الفهرس
Only 14 pages are availabe for public view

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Abstract

The present work studying the effect of Matcha tea powder on the biological, biochemical and histological changes of male white rats infected with obesity and the undesirable side effects accompanied obesity. Study the effect of different concentrations (2, 4 and 6%) of Matcha tea powder on improving the health status and estimating some other physiological properties such as (body weight, feed intake, food efficiency ratio. Some biochemical analysis such as liver and kidney functions, total cholesterol, triglycerides, HDL-c, LDL-c, VLDL-c, glucose level, total globulin, albumin and hematological parameters (RBC, WBC, Hb & Pl) in experimental rats. Also, determination of chemical composition, some active compounds such as total phenols, total flavonoids, antioxidant activity (DPPH) and identification of phenolic compounds using high performance liquid chromatography in matcha tea powder.
For this reason, a group of rats fed on high fat diet (HFD, 20% animal fat) for 4 weeks. Thirty adult male albino rats, Sprague Dawey Strain, 10 weeks age, weighing (140±10g) was used. Rats were distributed on 5 groups, 6 rats each. The first group was healthy rats fed on the basal diet, and the 2nd group obesity infected rats fed on fight fat diet. The 3rd, 4th and 5th groups, included rats fed on 2.0% , 4% and 6% Matcha green tea powder diets.
Feeding experimental continued for 28 days, then sacrificed. Biological and biochemical parameters evaluated and histopathological characteristics investigated.
The obtained results could be summarized in the following:
Chemical composition of Matcha tea powder:
1. The moisture, protein, fat, ash, fiber, carbohydrates and energy value contents of Matcha tea as dry weight (D/W) were 4.53, 17.95, 2.57, 5.97, 17.41, 51.57 and 319.33 Kcal/100g, respectively.
Total phenolics, total flavonoids and antioxidant activity content of Matcha tea powder:
1. The total phenolics, total flavonoids and antioxidant activity content of matcha tea powder were 745.53 mg GAE/gm, 657.75 mg rutin/g DM and 78.36 mg trolox/100gm., respectively.
Identification of phenolics compounds of Matcha tea powder:
1. The highest phenolics compounds of Matcha tea powder recorded for caffeine, epicatechin, catechin. The values were 142.31, 91.38, 36.38 mg/g, respectively.
2. While, the lowest value recorded for gallic acid, epigallocatechin and gallocatechin gallate. The values were 19.45, 18.51 and 4.26 mg/g DW, respectively.
3. On the other hand, ferulic acid p-coumaric acid and para-Hydroxy benzoic did not detected at these conditions.