الفهرس 
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Abstract
The aim of this study was to assess the level of the Platelet Derived Growth Factor and Vascular Endothelial Growth Factor in gingival Crevicular fluid following the use of a L-PRF alone or in combination with Collagen membrane in treatment of 2 and 3 walled vertical bone defects.
A secondary outcome was to evaluate the Clinical effect of using such collagen membranes with Platelet Rich Fibrin in the treatment of vertical bone defects.
A total number of 30 patients were enrolled in this study. Patients (18 females and 12 males; aged 28 to 49 years;) with 2 or 3 osseous wall interproximal defects in the premolar/molar areas (stage III, grade B) were recruited from the outpatient clinic of Oral Medicine, Periodontology and Oral Diagnosis department, Faculty of Dentistry, Ain Shams University. The three treatment modalities were randomly allocated (January 2021 to November 2021) by a predetermined computer generated randomization list (www.randomizer.org) into 4 equal groups;
➢ group I (control group) open flap debridement (OFD):
included intrabony defects treated by open flap debridement (OFD).
➢ group II Leucocyte-platelet rich fibrin (L-PRF): included
intrabony defects treated by leukocytes platelet rich fibrin (L- PRF).
➢ Group III Leucocyte-platelet rich fibrin + Collagen
membrane (L-PRF + CM): included intrabony defects treated by Leucocyte platelet rich fibrin & collagen membranes.
The primary outcomes included the biochemical analysis for the release profile of PDGF and VEGF at days 1, 3,5, 7, 11, 21, and
30. Secondary clinical outcomes were: plaque index (PI), gingival index (GI), probing depth (PD), clinical attachment level (CAL) while, radiographic measurements included cemento-enamel junction to the base of the defect (CEJ-BD) and cemento-enamel junction to the alveolar crest (CEJ-AC).
Regarding probing depth, no statistically significant change in mean or percentage of change at 6 month follow-up interval was found between L-PRF+CM and each of OFD and L-PRF groups where (p=0.617) and (p=0.325) respectively.
Comparison of mean Cal values between baseline and 6 month follow-up in all groups didn’t demonstrate any statistically significant difference. However, similar to mean probing depths, intragroup comparisons showed statistically significant difference within each group through out time.
Radiographic evaluation showed a statistically significant difference in in CEJ-BD at 6 month follow-up interval between group III (L-PRF+PM) and the other two groups in favor of the former where (p=0.001). No statistically significant difference was found between the other two groups.
At baseline and 6 month follow-up intervals, there was no statistically significant difference in CEJ-AC between groups 1, 2, and 3 where (p= 0.41 ). On the other hand, within each group, there was a statistically significant difference in CEJ-AC between (Baseline) and (After 6m) follow up intervals where groups 1, 2, and 3 calculated p= 0.001, 0.009, and 0.004 respectively.