الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
The new classification of Head and Neck Squamous Cell Carcinoma (HNSCC) according to their association with human papillomavirus (HPV) infection into HPV positive tumors with good prognosis and HPV negative tumors with a poor prognosis has changed the treatment strategy of HNSCCs dramatically worldwide. However, data concerning HPV infection in HNSCCs patients is still limited and there are no studies regarding HPV genotypes in HNSCC among Egyptians. Therefore, the aim of the current study is to investigate the frequency of HPV and to detect the most prevalent HPV genotypes in Egyptian patients with oral cavity and oropharyngeal squamous cell carcinoma. Ninety-two oral brushing samples (76 oral cavity and 16 oropharynx) were collected by cytobrushing in CYTO-fast solution. HPV-DNA was subjected to two different molecular techniques with the e-BRID System™ for automatic detection and genotyping and the conventional PCR for detection of HPV using consensus primers (MYO9/MYO11). SPSS software was used for statistical analysis. Out of the 92 cases assessed, HPV DNA was found in three samples only (3/92; 3.3%) and the HPV66 genotype was the only genotype detected by the e-BRID System™. The Results of this study may indicate that the HPV-associated HNSCC among Egyptian patients is very low and highlight the presence of HPV66 genotype in these malignancies. Other studies from different governorates in Egypt are still needed to determine the exact frequency of HPV infections in head and neck cancer.