الفهرس 
Studies on molecular detection of acinetobacter species from meat
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Abstract
Although food is very important for the human life, it may be life threatening. Foodborne diseases are spreading worldwide through the increasing rate of fresh and undercooked food consumption. Foodborne pathogens including many types of bacterial species. This study was conducted to determine; the prevalence of Acinetobacter species isolated from meat samples, its phenotypic characteristics, its antimicrobial resistance profile; and their genotypic characteristics. A total of 110 samples collected from chicken (n=50), beef (n=44), rabbit (n=10), and mutton (n=6) were examined bacteriologically. The suspected colonies were identified biochemically then tested for their antimicrobial resistance, biofilm formation, hemolytic activity then confirmed by PCR for some genes; rpoB, traT, fimH, and epsA. 9 Acinetobacter species (8.2%) were recovered where, 55 of examined samples were non-lactose fermenter with an incidence of 50%, 29 of examined samples were late lactose fermenter with an incidence of 26% and the rest of samples showed no growth or were lactose fermenter. The isolates showed high resistance to ceftriaxone, imipenem, ceftazidime and ticarcillin/Clavulanic acid in a percentage of 89%, 77.8%, 66.7% and 66.7 %, respectively. While, low resistance was found to Sulfamethazole/trimethoprim, doxycycline and amikacin in a percentage of 44.4%, 33.3% and 11.1%, respectively. However, the isolates showed no resistance to ciprofloxacin. All the isolates were MDR with MDRindex (more than 0.5), only one isolate was weak biofilm producer but, no isolate was able to the hemolysis of the sheep RBCS. 88.9% of the isolates expressed traT and fimH genes but, only 5.6% of the isolates could express epsA gene. It can be concluded that Acinetobacter species could be isolated from meat samples of animal origin in Egypt